In Vitro Anticancer Activity on 3T3, PC3, and HeLa Cell Line and GC-MS Assay of Oil Fractions of Alstonia Scholaris Flower Obtained by Column Chromatography

Kaneez Fatima, Shaukat Khalid, Kiran Qadeer, Hina Yasin, Hina Abrar, Raheela Bano, Adeel Arsalan, Rana Asif Hussain


Several plant-derived compounds are currently successfully employed in cancer treatment. Various studies have demonstrated the anticancer or cytotoxic potential of different extracts of Alstonia scholaris at the highest doses. This study aimed to analyze the anticancer potential of oil fractions obtained from Alstonia scholaris flower isolation. The oil fractions were characterized using gas chromatography–mass spectrometry (GC-MS) assay. The (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide) (MTT) colorimetric assay was used for 3T3, PC3, and HeLa cell line cytotoxic potential of oil fractions. The results showed no cytotoxic activity of oil fractions, i.e., Oil I and Oil II were at a minimum dose of 30 μg/ml. However, the GC-MS assay depicted saturated (palmitic acid, ethyl ester, caproic acid, stearic acid, ethyl ester, octacosanoic acid, etc.), unsaturated (oleic acid, 3-(octadecyloxy)propyl ester, ethyl oleate and some other hydrocarbons such as betulin, ethyl iso-allocholate, lupenyl acetate, phthalic anhydride, phytol, etc. Thus, the reported chemical constituents showed no significant anticancer effect in the scientific literature. This research suggested that Alstonia has no potential as an anticancer agent. The findings of this study provide insight into maintaining the optimum data for future aspects of plant-derived phytoconstituents with potential cytotoxic effects on 3T3, PC3, and HeLa cell lines.   


Keywords: Alstonia scholaris, GC-MS assay, MTT colorimetric assay, cytotoxicity.

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