In Vitro Antioxidant and Anti-Inflammatory Properties of Ethyl Acetate Extract Garcinia Tetranda Pierre Fruit Peel

Devyana Dyah Wulandari, Andreas Putro Ragil Santoso, Solehah Nur Cahyani, Nurul Chilmiyah, Ikke Erika, Novita Ramadani, Silvia Ramadani, Handayani, Arif Fadlan, Mardi Santoso

Abstract

The large genus Garcinia has encouraging bioactivity. However, the traits of many Garcinia species have not been studied thoroughly. This study was to analyze the antioxidant and anti-inflammatory activities of ethyl acetate extract of Wadung fruit skin in vitro. Antioxidant activity was determined using the ABTS method, while in vitro anti-inflammatory tests included inhibition of heat-induced hemolysis, hypotonic-induced hemolysis, protein denaturation, antiproteinase, and cyclooxygenase. The ethyl acetate extract of Wadung fruit skin showed very strong antioxidant activity as indicated by the IC5 value of 5,005, this was supported by the high content of phenolic and flavonoid compounds as indicated by the TPC and TFC values of 104.09 ± 1.66 mg GAE / g and 102.89 ± 1.59 mg QE / g, respectively. The results of the in vitro anti-inflammatory test also showed the potential to inhibit inflammation, as shown by the IC50 in the heat-induced hemolysis test of 1.65, hypotonic-induced hemolysis test of 3.53, protein denaturation test of 2.95, antiproteinase test of 4.61, and cyclooxygenase inhibition test of 2.02. Therefore, it can be concluded that the ethyl acetate extract of Wadung fruit skin has potential as a candidate for anti-inflammatory drug.

 

Keywords: Hemolysis, denaturation, Garcinia tetranda, antiproteinase

 

https://doi.org/10.55463/issn.1674-2974.51.10.7


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