Encapsulation of Ethanol Extract Sonneratia Alba Leaves with Inulin Matrix Coating as an Antioxidant Supplement
Abstract
Perepat (Sonneratia alba) is a mangrove species widely reported to have potential as a medicinal plant. Encapsulation in coating technology has been developed to prevent bioactive damage and to improve bioavailability. This study aimed to analyze the characteristics of ethanol extracts of Sonneratia alba (S. alba) leaves encapsulated using an inulin coating and to determine the encapsulation ability of ethanol extracts from perepat leaves. The highest total phenol and flavonoid extract levels were obtained in PKL at 171,880 mg GAE/g and 25,473 mg QE/g, respectively, in line with its antioxidant activity (IC50 3,544 μg/mL). The highest total phenol and flavonoid levels were obtained in PKL encapsulating 38.963 mg GAE/g and 5.583 mg QE/g, respectively, with an IC50 of 91.571 μg/mL. IR results of encapsulated ethanol extracts of S. alba leaves from four different regions showed the presence of C-H alkane functional groups, C-H alkene functional groups, C-H aromatic ring functional groups, O-H functional groups, hydrogen/phenol bond alcohols, C=C alkene functional groups, and C-O functional groups of alcohol/ether/carboxylic acids/esters. The IR spectrum confirms the cross-linking reaction and the formation of the Encapsule. SEM produces particles of various sizes, irregular surface structures, and deep hollows in walls. The results of in vitro bioavailability testing of ethanol extract encapsulation from perepat leaves with inulin coating of PKL showed an increase in the percentage of drug release in the pH buffer 1.2 from the previous minute to minute 120, which increased to 41.41%. In the pH buffer 7.4 from the last minute to minute 240, there was an increase in drug release, reaching 98.54%. This finding shows that encapsulation of the extract has good potential as a capsule supplement.
Keywords: antioxidant, bioavailability, encapsule, Sonneratia alba.
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