The Use of Honey as Anti-Oxidative Agent: Hatching Rate Embryo of Tor Soro after 48h Post-Cold Storage

Suci Lestari, Abinawanto, Anom Bowolaksono, Retno Lestari, Astari Dwiranti, Rudhy Gustiano, Anang Hari Kristanto


Honey as an anti-oxidative agent contains simple sugars (monosaccharides) such as fructose, glucose, and antioxidants that maintain motility, viability, and integrity of spermatozoa membranes in the preservation process. The use of honey as an antioxidant agent in sperm preservation varies between species due to differences in biophysical characteristics between cell types, and it is necessary to optimize the sperm preservation protocol for Mahseer (Tor soro) fish. Although the ability of honey as an antioxidant agent in the preservation of T. soro sperm has been proven through the analysis of abnormalities, viability, and sperm motility, the ability of post-preserved sperm fertility has rarely been reported. This study aims to evaluate sperm function after preservation by looking at the percentage of hatching rate of T. soro establishing a honey-optimized sperm preservation protocol on T. soro. Different concentrations of honey were investigated in postfertilization embryos by looking at the sperm quality of T. soro fish. For this purpose, the stripping technique is carried out by diluting the fish ringer solution and honey in a ratio of 1:10. After storage at 4oC for 48 hours, sperm is thawed at room temperature for 35 seconds and mixed with 100 eggs for fertilization. The percentage hatching rate of embryos was analyzed by ANOVA and Tukey tests. This study showed that honey affected the hatching rate of T. soro embryos after storage at 4oC (p< 0.05). The concentration of 1% honey resulted in the highest hatching rate of embryos with a total of 16.33 ± 1.35%.


Keywords: honey, anti-oxidative agent, sperm, cold storage, hatching rate.

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