Isolation and Characterization of Microcrystalline Cellulose from Alginate Residue of Brown Seaweed Sargassum Polycystum

Ilham Wahyu Pamungkas, Amir Husni, Siti Ari Budhiyanti

Abstract

Seaweed has great potential in the pharmaceutical field, developing into microcrystalline cellulose (MCC). MCC is a pharmaceutical solid dosage form as an excipient and is very suitable for manufacturing direct-printed tablets that function as binders and fillers. The purpose of this study was to isolate and characterize MCC from brown seaweed Sargassum polycystum and its alginate extraction residues through acid and enzyme hydrolysis methods. S. polycystum was prepared, and then alginate was extracted. The residue obtained from the alginate extraction and seaweed powder was then isolated to obtain a-cellulose and then hydrolyzed using 2.5 N HCl and cellulase. The characteristics of MCC tested were yield, solubility, water content, ash content, pH, Fourier Transform-Infra Red (FTIR) analysis, and X-Ray Diffraction. The results showed that MCC produced from different types of samples and isolated treatment of microcrystalline cellulose did not meet the requirements of the British Pharmacopeia. However, the results showed that MCC from alginate extraction residue with enzyme hydrolysis treatment was closer to the standard (Avicel PH 101) with a yield of 35.16 ± 9.02%, pH 6.60 ± 0.36, water content 2.50 ± 1.34%, ash content 5.55 ± 0.09%, and solubility 35.23 ± 10.57%. The results of the FTIR analysis test also showed typical peaks of cellulose, namely 3444.5 cm-1 for O-H and 2907.61 cm-1 for C-H, with a crystallinity degree of 74.73%.

 

Keywords: microcrystalline cellulose, alginate extraction residue, Sargassum polycystum, acid hydrolysis method, enzyme hydrolysis method.


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FAO (FOOD and AGRICULTURE ORGANIZATION). Global seaweeds and microalgae production, 1950–2019. World Aquaculture Performance Indicators (WAPI) factsheet. 2021. http://www.fao.org/3/ cb4579en/cb4579en.pdf

PUSPITA M., SETYAWIDATI N.A. R., STIGER-POUVREAU V., VANDANJON L., WIDOWATI I., RADJASA O.K., and BOURGOUGNON N. Indonesian Sargassum species bioprospecting: potential applications of bioactive compounds and challenge for sustainable development. Advances in Botanical Research, 2020, 95: 113-161. https://doi.org/10.1016/bs.abr.2019.12.002

MANTEU S.H., NURJANAH N., and NURHAYATI T. Characteristics of brown seaweeds Sargassum policystum and Padina minor from Pohuwato Water, Gorontalo. Jurnal Pengolahan Hasil Perikanan Indonesia, 2018, 21(3): 396-405. https://doi.org/10.17844/jphpi.v21i3.24709

VIJAY K., BALASUNDARI S., JEYASHAKILA R., VELAYATHUM P., MASILAN K., and RESHMA R. Proximate and mineral composition of brown seaweed from Gulf of Mannar. International Journal of Fisheries and Aquatic Studies, 2017, 5(5): 106-112. https://www.fisheriesjournal.com/archives/2017/vol5issue5/PartB/5-4-88-968.pdf

DIHARNINGRUM I.M., and HUSNI A. Acid and calcium alginate extraction method affected the quality of alginate from brown seaweed Sargassum hystrix J. Agardh. Jurnal Pengolahan Hasil Perikanan Indonesia, 2018, 21(3): 532-542. https://doi.org/doi.org/ 10.17844/jphpi.v21i3.24737

NOSYA M.A. Pembuatan mikrokristal selulosa dari tandan kosong kelapa sawit. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Lampung, Lampung, 2016.

EDISON, DIHARMI A., and SARI E.D. Characteristic of microcrystalline cellulose from red seaweed Eucheuma cottonii. Jurnal Pengolahan Hasil Perikanan Indonesia, 2019, 22(3): 483-489. https://doi.org/doi.org/ 10.17844/jphpi.v22i3.28946

RAHMI D., MARPAUNG M.T., AULIA R., PUTRI S., AIDHA N. N., and WIDJAJANTI R. Ekstraksi dan karakterisasi mikroselulosa dari rumput laut coklat Sargassum sp. sebagai bahan penguat bioplastik film. Jurnal Kimia dan Kemasan, 2020, 42(2): 57-65. DOI:10.24817/jkk.v42i2.6401

YOHANA C.A., SRIWIDODO S., and ABDASSAH M. Microcrystalline cellulose as pharmaceutical excipient. In book: Pharmaceutical Formulation Design - Recent Practices. IntechOpen, 2020: 1-22. DOI:10.5772/intechopen.88092

PRASETIA I.G.N.J.A., DEVIANA S., DAMAYANTI T., CAHYADI A., and WIRASUTA I.M.A.G. The effect NaOH concentration in delignification process on microcrystalline cellulose from green algae (Cladophora sp.) as the renewable marine product. Journal of Pharmaceutical Sciences and Community, 2018, 15(2): 68-71. https://doi.org/10.24071/jpsc.001026

TARCHOUN A.F., TRACHE D., and KLAPÖTKE T.M. Microcrystalline cellulose from Posidonia oceanica brown algae: Extraction and characterization. International Journal of Biological Macromolecules, 2019, 138: 837-845. https://doi.org/10.1016/j.ijbiomac. 2019.07.176

SARI L. Pengaruh konsentrasi asam klorida terhadap karakteristik mikrokristalin selulosa dari limbah padat industri agar-agar. Institut Pertanian Bogor, Bogor, 2017.

ROWE R.C., SHESKEY P. J., and QUINN M.E. Handbook of pharmaceutical excipients. 6th ed. Pharmaceutical Press, 2009: 506-509. https://adiyugatama.files.wordpress.com/2012/03/handbook-of-pharmaceutical-excipients-6th-ed.pdf

SURYADI H., SARI H.R., and ROSIKHOH D. Preparation of microcrystalline cellulose from water hyacinth powder by enzymatic hydrolysis using cellulase of local isolate. Journal of Young Pharmacists, 2017, 9(1): S19-S23. https://doi.org/10.5530/ jyp.2017.1s.6

HUSNI A., SUBARYONO, PRANOTO Y., TAZWIR, and DAN USTADI. Development of extraction method from Sargassum sp. as thickening. Agritech, 2012, 32(1): 1-8. https://doi.org/10. 22146/agritech. 9649

GUSRIANTO P., ZULHARMITA, and RIVAI H. Preparation and characterization of microcrystalline cellulose from waste of sawdust. Jurnal Sains dan Teknologi Farmasi, 2011, 16(2): 180-188. http://repo.unand.ac.id/1707/1/63-129-1-SM%2520JSTF%252016%25282%2529%25202011.pdf

AOAC (ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTRY). Official method of analysis of the association of official analytical chemistry. AOAC International, Washington DC (US). Retrieved August 24, 2021 from https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/

SUNARDI, LESTARI A., JUNAIDI A.B., and ISTIKOWATI W.T. Isolation of microcrystalline cellulose from medang wood (Neolitsea latifolia). Konversi, 2019, 8(2): 7-14. https://doi.org/10.20527/k.v8i2.6881

PUTRI C.B., and SURYADI H. Effect of beta-glucosidase inhibitor from lichen extract in microcrystalline cellulose preparation from water hyacinth (Eichhornia crassipes). Pharmacognosy Journal, 2019, 11(6): 119-1203. https://doi.org/10.5530/pj.2019.11.186

FONG J., IBRAHIM A., ABDULAH M., and SAM S.T. Optimizing yield of microcrystalline cellulose from empty fruit bunch via hydrolysis using ionic liquid. IOP Conference Series: Materials Science and Engineering, 2018, 429: 012060. https://doi.org/10.1088/1757-899X/429/1/012060

NURKHANIFAH S.I., and HUSNI A. The effect of sodium carbonate extraction ratio on the quality of alginate from Sargassum muticum. Jurnal Teknosains, 2020, 10(1): 10-18. https://doi.org/ 10.22146/teknosains.41982

MAULANA S., FADIL A., and DRASTINIWATI. Kinetika reaksi demineralisasi isolasi kitin dari cangkang ebi. Jurnal Online Fakultas Teknik, 2017, 4(2): 1-5. https://jom.unri.ac.id/index.php/JOMFTEKNIK/article/view/15187

PAWEKA Y. M. Analisis natrium dalam air laut di sekitar pesisir Pantai Papua dengan metode spektroskopi serapan atom. Indonesia Journal of Applied Science, 2017, 7(2): 19-24. https://doi.org/10.24198/ijas.v7i2.14987

OCTAVIANA M. Optimasi preparasi mikrokristalin selulosa dari sekam padi menggunakan H202 dan NaOCl untuk sintesis carboxymethyl cellulose. Universitas Negeri Semarang, Semarang, 2017.

SANTOS A.A.D., and WAHYUNIGTYAS D. Karakterisasi mikrokristalin selulosa dari daun jambu biji (Psidium guajava L) sebagai eksipien tablet obat diare. Jurnal Inovasi Proses, 2019, 4(1), 1-7. https://ejournal.akprind.ac.id/index.php/JIP/article/view/1870/1459

AMALIA N. Pengaruh konsentrasi HCl terhadap delignifikasi serbuk pelepah kelapa sawit. Fakultas Farmasi, Universitas Sumatra Utara, Medan, 2018.

BI Z., LAI B., ZHAO Y., and YAN L. Fast disassembly of lignocellulosic biomass to lignin and sugars by molten salt hydrate at low temperature for overall biorefinery. American Chemical Society Omega, 2018, 3(3), 2984-2993. https://doi.org/10.1021/acsomega.8b00057

TAMAHEANG T., MAKAPEDUA, DAISY M., and BERHIMPON S. Kualitas rumput laut merah (Kappaphycus alvarezii) dengan metode pengeringan sinar matahari dan cabinet dryer, serta rendemen semi-refined carrageenan. Media Teknologi Hasil Perikanan, 2017, 5(2): 58-63. DOI: https://doi.org/10.35800/mthp.5.2.2017.14925

ANSHARULLAH A., SAENUDDIN N.M.A., FARADILLA R.F., ASRANUDIN A., ASNIAR A., and NURDIN M. Production of microcrystalline cellulose from tapioca solid waste: effect of acid concentration on its physicochemical properties. Jurnal Kimia Sains dan Aplikasi, 2020, 23(5): 147-151. https://doi.org/10.14710/jksa.23.5.147-151

RIVAI H., RAMDANI R., ANDAYANI R., ARMIN F., and DJAMAAN A. Preparation and characterization of microcrystalline cellulose from rice straw using chemical and enzymatic techniques. International Research Journal Pharmacy, 2019, 10(7): 27-32.DOI:10.7897/2230-8407.1007213

SARI N.W., MISKAH Y.F., and ANJAS W. Analisis fitokimia dan gugus fungsi dari ekstrak etanol pisang goroho merah (Musa Acuminate (L). Indonesian Journal of Biotechnology and Biodiversity, 2018, 2(1): 30-34. https://ejurnal.esaunggul.ac.id/index.php/bio/article/view/2520

YUGATAMA A., MAHARANI L, PRATIWI H., and IKADITYA L. Uji karakteristik mikrokristalin selulosa dari nata de soya sebagai eksipien tablet. Farmasains, 2015, 2(6): 269-274. https://www.researchgate.net/publication/341591085_Uji_Karakteristik_Mikrokristalin_Selulosa_Dari_Nata_De-Soya_Sebagai_Eksipien_Tablet

KIAN L.K., JAWAID M., ARIFFIN H., and ALOTHMAN O.Y. Isolation and characterization of microcrystalline cellulose from roselle fibers. International Journal of Biological Macromolecules, 2017, 103: 931-940. https://doi.org/10.1016/j.ijbiomac.2017.05.135

LIU Y., LIU A., IBRAHIM S.A., YANG H., and HUANG W. Isolation and characterization of microcrystalline cellulose from pomelo peel. International Journal of Biological Macromolecules, 2018, 111: 717-721. https://doi.org/10.1016/j.ijbiomac. 2018.01.098.

LESTARI A.S., and SARTIKA D. Preparasi dan karakterisasi nanopartikel Fe3O4 menggunakan metode kopresipitasi. Jurnal Teknologi Technoscientia, 2018, 11(1): 7-10. https://doi.org/10.34151/technoscientia.v11i1.109


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