Gorontalo Local Chicken Diversity Based on IGF-1 (Insulin-Like Growth Factor 1) Gene Analysis
Abstract
This study aims to examine the diversity of local Gorontalo chicken using the IGF-1 (Insulin-Like Growth Factor 1) gene marker. The sampling was conducted in six different areas, namely Gorontalo City, North Gorontalo (Gorut), Bone Bolango (Bonebol), Boalemo, Pohuwato, and Gorontalo Regency (Kabgor). Furthermore, conventional real-time PCR methods were used, and the sequencing was conducted through the obtained product. The results were analyzed using MEGA 11 software, and the data were genetic distance based on nucleotide and protein composition and phylogeny tree. All samples showed high DNA purity (A260/A280) by 1.910-1.925, and the DNA concentration obtained was 82.10 – 82.60 mL. The real-time PCR test showed the Ct value of 18.20 – 18.50, while the melting temperature or Tm is in the range of 81.20 – 82.10°C. The PCR product sequencing and MEGA 11 analysis showed that the farthest genetic distance between chicken from Gorontalo City (City) and Boalemo Regency was 0.8584. The closest genetic distance is from North Gorontalo Regency (Gorut) by 0.0409. In conclusion, nucleotide and protein composition and phylogenetic tree analysis can be used to examine the diversity of local Gorontalo chicken using IGF1 gene markers by looking at genetic distance. The novelty of this study was that we identified the diversity of local Gorontalo chicken using the IGF-1 gene marker.
Keywords: insulin-like growth factor, genetic distance, Gorontalo local chicken.
Full Text:
PDFReferences
SOPHIAN A., ABINAWANTO, NISA U.C., and FADHILLAH. Morphometric analysis of Gorontalo (Indonesia) native chickens from six different regions. Biodiversitas, 2021, 22(4): 1757-1763, DOI: 10.13057/bio div/d220420.
ABINAWANTO, SOPHIAN A., LESTARI R., BOWOLAKSONO A., EFENDI P.S., and AFNAN R. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas, 2019, 20(7): 2004-2010. DOI: 10.13057/bio div/d200729.
PENG W., YANG H., CAI K., ZHOU L., TAN Z., and WU K. Molecular identification of the Danzhou chicken breed in China using DNA barcoding. Mitochondrial DNA Part B: Resources, 2019, 4(2): 2459-2463. DOI: 10.1080/23802359.2019.1638321.
CUI H., IBTISHAM F., XU C., HUANG H., and SU Y. DNA barcoding of Chinese native chicken breeds through COI gene. Thai J. Vet. Med., 2017, 47(1): 123-129.
KARP-TATHAM E., KÜSTER T., ANGELOU A., PAPADOPOULOS E., NISBET AL.J., XIA D., TOMLEY F.M., and BLAKE D.P. Phylogenetic Inference Using Cytochrome C Oxidase Subunit I (COI) in the Poultry Red Mite, Dermanyssus gallinae in the United Kingdom Relative to a European Framework. Frontiers in Veterinary Science, 2020, 7(8): 1-13. DOI: 10.3389/fvets.2020.00553.
DAVE A.R., CHAUDHARY D.F., MANKAD P.M., KORINGA P.G., and RANK D.N. Genetic diversity among two native Indian chicken populations using cytochrome c oxidase subunit I and cytochrome b DNA barcodes. Veterinary World, 2021, 14(5): 1389-1397. DOI: 10.14202/vetworld.2021.1389-1397.
OKUMU O.N., NGERANWA J.J.N., BINEPAL Y.S., KAHI A.K., BRAMWEL W.W., ATEYA O., and WEKESA F.C. Genetic diversity of indigenous chickens from selected areas in Kenya using microsatellite markers. Journal of Genetic Engineering and Biotechnology, 2017, 15(2): 489-495. DOI: 10.1016/j.jgeb.2017.04.007.
MALONE D.K., WEIGEND S., SCHMITT A.O., WEIGEND A., REIMER C., and SIMIANER H. Genetic diversity in global chicken breeds in relation to their genetic distances to wild populations. Genetics Selection Evolution, 2021, 53(1): 1-11. DOI: 10.1186/s12711-021-00628-z.
ROH H.J., KIM S.-C., CHO CH.-Y., LEE J., JEON D., KIM D.-K., KIM K.-W., AFRIN F., KO Y.-G., LEE J.-H., BATSAIKHAN S., HEGAY T.S.S., KONGVONGXAY S., GORKHALI N.A., THI L.A.N., THAO T.T.T., and MANIKKU L. Estimating genetic diversity and population structure of 22 chicken breeds in Asia using microsatellite markers. Asian-Australasian Journal of Animal Sciences, 2020, 33(12): 1896-1904. DOI: 10.5713/ajas.19.0958.
SABRY A., MOHAMED A.A., and HASSEN M. Genetic Diversity of Indigenous Chicken (Gallus Gallus domesticus) from Ecozones of Egypt and Kingdom of Saudi Arabia. Open Journal of Applied Sciences, 2021, 11(6): 775-787. DOI: 10.4236/ojapps.2021.116057.
ABINAWANTO, ZULISTIANA T., LESTARI R., DWIRANTI A., and BOWOLAKSONO A. The genetic diversity of ayam ketawa (Gallus gallus domesticus, linneaus, 1758) in bangkalan district, madura island, Indonesia. Biodiversitas, 2021, 22(6): 3145-3155. DOI: 10.13057/biodiv/d220617.
MUSTOFA F., FATHONI A., SARI A.P.Z.N.L., SASONGKO H., and MAHARANI D. Bodyweight and body size measurement of five Indonesian local chicken. IOP Conference Series: Earth and Environmental Science, 2021, 788(1). DOI: 10.1088/1755-1315/788/1/012016.
DARYONO B.S., MUSHLIH M., and PERDAMAIAN A.B.I. Crowing sound and inbreeding coefficient analysis of Pelung chicken (Gallus Gallus domesticus). Biodiversitas, 2021, 22(5): 2451-2457. DOI: 10.13057/bio div/d220501.
BUGIWATI S.R.A., DAGONG M.I.A., and TOKUNAGA T. Crowing characteristics of native singing chicken breeds in Indonesia. IOP Conference Series: Earth and Environmental Science, 2020, 492(1). DOI: 10.1088/1755-1315/492/1/012100.
ARLINA F., RUSFIDRA, ANDRIANO D., and SUMATRI C. Short communication: The type and sound diversity of kukuak balenggek chicken (gallus gallus domesticus) reared in West Sumatra, Indonesia. Biodiversitas, 2020, 21(5): 1914-1919. DOI: 10.13057/bio div/d210518.
ABINAWANTO, SOPHIAN A., EFFENDI P., and SISWANTINING T. Short communication: Variation in vocal cord morphometric characters among dangdut type and the slow type gaga chicken. Biodiversitas, 2018, 19(5): 1902-1905. DOI: 10.13057/bio div/d190542.
ABINAWANTO A., and EFFENDI P.S. The bioacoustics analysis and the morphometric study of the gaga's chicken (Ayam Ketawa) from Pinrang and Kebayoran Lama. AIP Conference Proceedings, 2018, 2023. DOI: 10.1063/1.5064134.
SOPHIAN A., PURWANINGSIH R., MUINDAR, IGIRISA E., and AMIRULLAH M. Short Communication : Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples. Asian Journal of Tropical Biotechnology, 2021, 18(1): 28-31. DOI: 10.13057/biotek/c180103.
SOPHIAN A. Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian Journal of Natural Product Biochemistry, 2021, 19(1): 21-24. DOI: 10.13057/biofar/f190104.
MU’IN M.A., SUPRIYANTONO A., and UHI D.H.T. Insulin-like Growth Factor-1 gene polymorphism and its effect on the local chicken growth. Indonesian Journal of Animal and Veterinary Science, 2009, 14(4): 289-295.
HELD P.G. Nucleic Acid Purity Assessment using A 260 / A 280 Ratios. Bio Tek Application Note, 2001, 3: 1-5.
MATLOCK B. Assessment of Nucleic Acid Purity. Technical Note, 2015, 52646.
SOPHIAN A., PURWANINGSIH R.M., IGIRISA E.P.J., and AMIRULLAH M.L. Detection of Salmonella typhimurium ATCC 14028 in Powder Prepared Traditional Medicines Using Real-Time PCR. Borneo Journal of Pharmacy, 2021, 4(3): 178-183.
SOPHIAN A., PURWANINGSI R., LUKITA B.L., and NINGSIH E.C. Detection of Salmonella typhimurium ATCC 14028 in supplement health product liquid preparation using Real-Time PCR (qPCR). Biofarmasi Journal of Natural Product Biochemistry, 2020, 18(2): 61-65. DOI: 10.13057/biofar/f180202.
BABAYI M.S., SEYEDABADI H., GORBANI A., and ZARGHAMI N. IGF-I Gene Polymorphism and its Associations with Some Growth traits in West-Azerbaijan Native chicken using PCR-RFLP Techniques. Bulletin of Environment, Pharmacology and Life Sciences , 2014, 3(5): 29-32.
KADLEC J., HOSNEDLOVÁ B., ŘEHOUT V., ČÍTEK J., VEČEREK L., and HANUSOVÁ L. Insulin-like growth factor-I gene polymorphism and its association with growth and slaughter characteristics in broiler chickens. Journal of Agrobiology, 2012, 28(2): 157-163. DOI: 10.2478/v10146-011-0017-4.
ALI A., JAVED K., ALI A., AKRAM M., DAWOOD M., and SALEEM A.H. Polymorphism of insulin-like growth factor-1 gene and its association with growth rate in desi chicken of Pakistan. Journal of Animal and Plant Sciences, 2016, 26(3): 858-861.
ZHOU H., MITCHELL A.D., MCMURTRY J.P., ASHWELL C.M., and LAMONT S.J. Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens. Poultry Science, 2005, 84(2): 212-219. DOI: 10.1093/ps/84.2.212.
GASPARINO E., VOLTOLINI D.M., DEL VESCO A.P., GUIMARÃES S.E.F., DO NASCIMENTO C.S., and DE OLIVEIRA NETO A.R. IGF-I, GHR and UCP mRNA expression in the liver and muscle of high- and low-feed-efficiency laying Japanese quail at different environmental temperatures. Livestock Science, 2013, 157(1): 339-344. DOI: 10.1016/j.livsci.2013.06.028.
TABASSUM KHAN N. MEGA - Core of Phylogenetic Analysis in Molecular Evolutionary Genetics. Journal of Phylogenetics and Evolutionary Biology, 2017, 5(2): 2-5. DOI: 10.4172/2329-9002.1000183.
KUMAR S., STECHER G., and TAMURA K. MEGA 7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 2016, 33(7): 1870-1874. DOI: 10.1093/molbev/msw054.
JIA J. AHMED I., LIU L., LIU Y., XU Z., DUAN X., LI Q., DOU T., GU D., RONG H., WANG K., LI Z., TALPUR M.Z., HUANG Y., WANG S., YAN S., TONG H., ZHAO S., ZHAO G., TE PAS M.F.W., and SU Z. Selection for growth rate and body size have altered the expression profiles of somatotropic axis genes in chickens. PLoS One, 2018, 13(4): 1-20. DOI: 10.1371/journal.pone.0195378.
LAN ANH N.T., KUNHAREANG S., and DUANGJINDA M. Association of chicken growth hormones and insulin-like growth factor gene polymorphisms with growth performance and carcass traits in Thai broilers. Asian-Australasian Journal of Animal Sciences, 2015, 28(I12): 1686-1695. DOI: 10.5713/ajas.15.0028.
HOSNEDLOVA B. VERNEROVA K., KIZEK R., BOZZI R., KADLEC J., CURN V., KOUBA F., FERNANDEZ C., MACHANDER V., and HORNA H. Associations between IGF1, IGFBP2 and tgfß3 genes polymorphisms and growth performance of broiler chicken lines. Animals, 2020, 10(5): 1-24. DOI: 10.3390/ani10050800.
Refbacks
- There are currently no refbacks.
