Water Infusion of IR-Bagendit Rice Leaves from Various Locations in Central Java as a Candidate Material to Prevent a Heavy Metal Exposure
Abstract
The use of heavy metals in many industries provides multiple advantages but can pollute the environment and cause health problems that endanger living beings’ lives. Moreover, the clinical use of chelating materials causes discomfort and neurotoxicity. Therapy for heavy metal poisoning can be implemented using chelating materials, such as DMSA, BAL, DPCN, and EDTA. Water infusion of IR-Bagendit rice leaves contains metallothionein proteins, potentially becoming a candidate material to prevent heavy metal exposure. The metallothionein protein can bind and detoxify heavy metals in covalent. IR-Bagendit is plentifully found in Central Java. However, no study has investigated the metallothionein protein content of IR-Bagendit leaves from different locations to prevent heavy metals. This study aimed to determine the metallothionein protein content of IR-Bagendit leaves from Boja, Batang, Blora, and Weleri, Jawa Tengah, Indonesia. The research sample was the rice leaves aged four to six weeks from Boja, Batang, Blora, and Weleri. The study collected five snippets from each region. The collected rice leaves were made in infusion and examined using the ELISA method. This study revealed that the metallothionein protein levels of IR-Bagendit leaves, sequentially from the highest to lowest, are from Blora (380.636 ng/L), Weleri (252.189 ng/L), Batang (121.748 ng/L), and Boja (28.832 ng/L). The study concludes that the IR-Bagendit from Blora has the highest metallothionein protein content and potentially becomes a material candidate to prevent heavy metal exposure.
Keywords: water infusion of IR-Bagendit rice leaves, heavy metals, candidate material.
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