Application of Mean Centering Ratio and Multiple Wavelength Methods for Determination of Four Component Level in Multivitamin Syrup by Ultraviolet Spectrophotometry

Muchlisyam, Sandra Monica


The significance and logic of simultaneous determination of vitamin B1, B2, B6, and niacin levels in multivitamin syrup using the mean centering ratio (MCR) method and the multiple wavelength method (MWM), without separation. This study aims to determine the use of the divisor factor in the mean centering ratio method and use five different wavelengths to measure multiple wavelengths. The research methods are carried out using methanol-water as a solvent, triple divisor for the MCR method, and five different wavelengths for MWM. The test of validation parameters is linearity, accuracy, precision, detection, and quantitation. The determination of vitamin B1, B2, B6 and niacin levels in multivitamin syrup for MCR method are (101.44 ± 4.35)%, (100.19 ± 4.09)%, (98.75 ± 0.47)%, and (100.75 ± 1.06)% respectively and for Multiple Wavelength method are (98.05 ± 1.28)%, (99.04 ± 1.17)%, (99.02 ± 1.00)%, and (99.61 ± 1.17)% respectively. The result for validation in MCR method with the parameter: linearity, accuracy, precision, LOD, and LOQ according to USP 43 requirement. The Multiple Wavelength method has a good validation with parameter accuracy and coefficient of variation (CV). The divisor factor system of the Mean Centering Ratio method and five different wavelengths for multiple Wavelengths can be used for the determination of vitamin B1, B2, B6, and niacin levels in multivitamin syrup and fulfilled the validation of the parameters.


Keywords: multivitamin syrup, determination, mean centering ratio, multiple wavelength, spectrophotometric.

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