Date Palm Extract Effect on Quality of Mahseer Fish (Tor Soro) Spermatozoa after Frozen Storage
Abstract
Tor soro is one of the endemic fishes used in traditional ceremonies. Its natural population is threatened with extinction due to environmental damage and overfishing. One of the efforts to overcome the population decline is carried out through the sperm cryopreservation process, which is influenced by a factor called cryoprotectant. Generally, cryoprotectants such as date palm extract are useful for protecting spermatozoa against damage during this process, and they are selected with natural ingredients to reduce toxicity effects. Therefore, this research aims to evaluate the effect of date palm extract with a combination of 10% methanol on spermatozoa quality (motility, viability, and abnormalities) and the percentage of T. soro fertility at 48 hours after cryopreservation. The extract concentrations used were 0%, 5%, 10%, 15%, 20%, and 25%, the dilution ratio was 1:10, while storage was carried out in the freezer at -10 °C. The results of the one-way ANOVA test showed that the various concentrations with 10% methanol had a significant (P <0.05) effect on the spermatozoa motility, abnormalities, and fertilization ability. Hence, the combination of 10% methanol and 10% date palm extract is the optimum concentration to maintain the 81.29 ± 1.01% highest motility, 21.5 ± 1.29% lowest abnormality, and 88.50 ± 1.73% highest fertility.
Keywords: cryopreservation, date palm extract, fertilization, Masheer fish.
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