Potential CRISPR-Cas9 Associated Vector Lentivirus for CCR5 Gene Silencing On CD34+ Hematopoetic Cells Intermediate HIV-1 Resistance

Nurul Hidayah, Yuna Annisa Salsabila, Fitriana Kurniasari Solikhah, Rifaatul Laila Mahmudah


Potential of CRISPR / Cas9 encapsulated lentivirus vector for silencing the CCR5 gene on CD34+ hematopoietic cells mediating HIV-1 resistance. CRISPR-Cas9 and modified dual guide RNA encapsulated by lentivirus vector can silence CCR5 gene CD34+ hematopoietic cells. The purpose of this research was to determine the use of CRISPR-Cas9 in vivo using hematopoietic stem cells so that it can be a therapeutic modality with the aim of resistance to HIV-1. CRISPR-Cas9 has a specific target on the DNA sequence encoded by the modified guide RNA. This specificity allows CRISPR-Cas9 to reduce the risk of off-targeting the DNA of the host cell. Silencing of the CCR5 gene on CD34+ hematopoietic cells can make its derivative cells resistant to HIV because the CCR5 gene is not expressed as CCR5 chemokines. As a result, the host cell will become immune to HIV infection.


Keywords: CCR5, CRISPR/Cas9, Hematopoietik cell CD34+, Lentivirus vector.



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