USP13 Restrains Apoptosis of Liver Cancer Cells Induced by NDV
Abstract
Newcastle Disease Virus is an avian virus with strong replication ability,which can specifically identify and kill tumor cells. When liver cancer cells are infected with NDV,the apoptosis signaling pathway is activated,inducing apoptosis. USP13 (Ubiquitin Specific Protease 13) is a member of the ubiquitination enzyme family,and deubiquitylate and stabilize PTEN. The expression of USP13 is obviously decreased when NDV infected Huh7 or HLCZ01 cells. USP13 is overexpressed in NDV-infected Huh7 or HLCZ01 cells,which significantly restrains apoptosis. Conversely,knockdown USP13 significantly enhances apoptosis. Further studies revealed the underlying mechanism that USP13 restrains NDV-induced apoptosis. Although overexpression of USP13 doesn't affect NDV replication in two types of liver cancer cells,USP13 can upregulate an important molecule Bcl-2,thereby inhibiting apoptosis. USP13 plays an important role in NDV-induced apoptosis. At the same time,a new theory basis for the oncolytic treatment of liver cancer is provided.
Keywords: USP13, NDV, HCC, apoptosis, c-PARP, Bcl-2, cell, enzyme
Full Text:
PDFReferences
GRANDHI M S,KIM A K,RONNEKLEIV -KELLY S M,et al. Hepatocellular carcinoma: from diagnosis to treatment [J]. World Journal of Hepatology,2015,7(8):74—85.
MAHMOUD M,SAMOILOV D,WEINBERG G,et al. Treatment of hepatocellular carcinoma(HCC)using multi-modality therapy ina community based setting [J]. Am J Gastroenterol,2015,110: S918—S9199.
FUJIWARA T. Targeted oncolytic adenovirus for human cancer therapy:gene -based therapies for cancer1970 [M]. Gene -Based Therapies for Cancer,2010:79—93.
SINKOVICS J G,HORVATHJ C. Newcastle disease virus(NDV): brief history of its oncolytic strains [J]. Journal of Clinical Virology,2000,16(1):1—15.
PEINA-LAUS N. Wnt signal transduction pathway and apoptosis:a review[J]. Cancer Cell International,2010,10(1):22—26.
YANG D R,ZUO C H,WANG X H,et al. Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line [J]. Proceedings of the National Academy of Sciences of the United States of America,2014,111(13):1264— 1273.
FAN X,LU H,CUI Y,et al. Overexpression of p53 delivered using recombinant NDV induces apoptosis in glioma cells by regulating the apoptotic signaling pathway [J]. Experimental & Therapeutic Medicine,2018,15(5):4522—4530.
WANG H G,MIYASHITA T,TAKAYAMA S,et al. Apoptosis regulation by interaction of Bcl-2 protein and Raf-1 kinase[J]. Oncogene,1994,9(9):2751—2756.
KIM K W,LEE J M,CHOI B I. Assessment of the treatment response of HCC[J]. Abdom Imaging,2011,36(3):300—314.
LIU N,LONG Y,LIU B,et al. ISG12a mediates cell response to Newcastle disease viral infection[J]. Virology,2014,462/463(1): 283—294.
ZHANG J S,ZHANG P J,WEI Y K,et al. Deubiquitylation and stabilization of PTEN by USP13 [J]. Nature cell biology,2013,15 (12):1486—1494.
Refbacks
- There are currently no refbacks.