The Inhibitory Activity of Medinilla Syrup Butanolic Extract against Candida Albicans ATCC 10231

Marcel Kurniadi, Agus Limanto, Kris Herawan Timotius


Vulvovaginal candidiasis is a fungal infection with the leading cause being Candida albicans. It can interfere with women's fertility of reproductive age. Eradication of the vaginal C. albicans can enhance women's fertility. In this case, herbal materials that have antifungal activity may help to reduce the population of C. albicans. One of the prosperous plants is Medinilla speciosa. This plant grows in the area of Colo, Mount Muria, Kudus, Indonesia. As an ornamental plant, the local community traditionally believes that using Medinilla speciosa enhances female fertility. Moreover, Medinilla syrup is available locally. Therefore, this study aims to determine whether Medinilla syrup can inhibit the growth of C. albicans. In this experiment, Medinilla syrup was macerated with butanol. The inhibition zone method, the minimum inhibitory concentration (MIC) method, and the real-time planktonic growth in the personal bioreactor (RTS BioSan) were used. The MIC of the butanoic extract of Medinilla syrup was 8.44 mg/mL. The real-time planktonic growth showed that the extract added into the growth tubes significantly decreased the growth rate and yields of C. albicans. Medinilla syrup contains bioactive compounds that inhibit the planktonic growth but not the biofilm-growth of C. albicans.


Keywords: Candida albicans, Medinilla speciosa, parijotho, fertility.

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FORNARI G., VICENTE V.A., GOMES R.R., MURO M.D., PINHEIRO R.L., FERRARI C., HERKERT P.F., TAKIMURA M., CARVALHO N.S., and QUEIROZ-TELLES F. Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis. Brazilian Journal of Microbiology, 2016, 47(2): 373-380. DOI: 10.1016/j.bjm.2016.01.005.

SASANI E., RAFAT Z., ASHRAFI K., SALIMI Y., ZANDI M., SOLTANI S., HASHEMI F., and HASHEMI S.J. Vulvovaginal candidiasis in Iran: A systematic review and meta-analysis on the epidemiology, clinical manifestations, demographic characteristics, risk factors, etiologic agents and laboratory diagnosis. Microbial Pathogenesis, 2021, 154: 104802. DOI: 10.1016/j.micpath.2021.104802.

CASTRILLÓN-DUQUE E.X., SUÁREZ J.P., and CARDONA MAYA W.D. Yeast and Fertility: Effects of In Vitro Activity of Candida spp. on Sperm Quality. Journal of Reproduction & Infertility, 2018, 19(1): 49-55.

DE BERNARDIS F., GRAZIANI S., TIRELLI F., and ANTONOPOULOU S. Candida vaginitis: virulence, host response and vaccine prospects. Journal of Medical Mycology, 2018, 56(11): 26-31.

CHEN Y., BRUNING E., RUBINO J., and EDER S.E. Role of female intimate hygiene in vulvovaginal health: Global hygiene practices and product usage. Women's Health (London), 2017, 13(3): 58-67. DOI: 10.1177/1745505717731011.

CAUCHIE M., DESMET S., and LAGROU K. Candida and its dual lifestyle as a commensal and a pathogen. Research in Microbiology, 2017, 168(9-10): 802-810.

HANUM A.S., PRIHASTANTI E., and JUMARI. Ethnobotany of utilization, role, and philosophical meaning of parijoto (Medinilla, spp) on Mount Muria in Kudus Regency, Central Java. AIP Conference Proceedings, 2017, 1868(1): 090018.

ASTUTTIK P.Y.R., and VIFTA R.L. Perbandingan aktivitas antifungi ekstrak etanol 70% dan 96% buah parijoto (medinilla speciosa) terhadap Candida albicans. Journal of Holistics and Health Science, 2021, 3(1): 32-41.

WATSON R.R. Bioactive foods in promoting health: probiotics and prebiotics. Britania Raya: Elsevier Science, 2016.

KOCHAN K., LAI E., RICHARDSON Z., NETHERCOTT C., PELEG A.Y., HERAUD P., and WOOD B.R. Vibrational Spectroscopy as a Sensitive Probe for the Chemistry of Intra-Phase Bacterial Growth. Sensors (Basel, Switzerland), 2020, 20(12): 34-52. DOI: 10.3390/s20123452.

ALVENDAL C., MOHANTY S., BOHM-STARKE N., and BRAUNER A. Anti-biofilm activity of chlorhexidine digluconate against Candida albicans vaginal isolates. PLoS One, 2020, 15(9): e0238428. DOI:

SAJJADI S.T., SABOORA A., and MOHAMMADI P. Comparison of aglycon and glycosidic saponin extracts of Cyclamen coum tuber against Candida spp. Current Medical Mycology, 2016. 2(2): 40-44. DOI:

USTUN O., BERRIN O., and BAYKAL T. Bioactivities of Ethanolic Extract and its Fractions of Cistus laurifolius L. (Cistaceae) and Salvia wiedemannii Boiss. (Lamiaceae) Species. Pharmacognosy Magazine, 2016, 12(1): 82-85. DOI: 10.4103/0973-1296.176125.

PEREIRA J.V., FREIRES I.A., CASTILHO A.R., DA CUNHA M.G., DA SILVA ALVES H., and ROSALEN P.L. Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of action against Candida albicans. Pharmaceutical Biology, 2016, 54(10): 2312-2319. DOI: 10.3109/13880209.2016.1155629.

ALAYANDE K.A., POHL C.H., and ASHAFA A.O.T. Time-kill kinetics and biocidal effect of Euclea crispa leaf extracts against microbial membrane. Asian Pacific Journal of Tropical Medicine, 2017, 10(4): 390-399. DOI:

RÓŻALSKA B., SADOWSKA B., ŻUCHOWSKI J., WIĘCKOWSKA-SZAKIEL M., BUDZYŃSKA A., WÓJCIK U., and STOCHMAL A. Phenolic and Nonpolar Fractions of Elaeagnus rhamnoides (L.) A. Nelson Extracts as Virulence Modulators-In Vitro Study on Bacteria, Fungi, and Epithelial Cells. Molecules, 2018, 23(7). DOI: 10.3390/molecules23071498.

dE SOUSA M.B., JÚNIOR J.O., BARBOSA W.L., DA SILVA VALÉRIO E., DA MATA LIMA A., DE ARAÚJO M.H., MUZITANO M.F., NAKAMURA C.V., DE MELLO J.C., and TEIXEIRA F.M. Pyrostegia venusta (Ker Gawl.) Miers Crude Extract and Fractions: Prevention of Dental Biofilm Formation and Immunomodulatory Capacity. Pharmacognosy Magazine, 2016, 12(2): 218-222. DOI: 10.4103/0973-1296.182150.

DOS SANTOS ALVES C.F., BONEZ P.C., DE SOUZA M.E., DA CRUZ R.C., BOLIGON A.A., PIANA M., BRUM T.F., ROSSI G.G., JESUS R.D., GRANDO T.H., MONTEIRO S.G., ANRAKU DE CAMPOS M.M., GIONGO J.L., and VIANNA SANTOS R.C. Antimicrobial, antitrypanosomal and antibiofilm activity of Equisetum hyemale. Microbial Pathogenesis, 2016, 101: 119-125. DOI: 10.1016/j.micpath.2016.11.008.

YOUNUS M., HASAN M.M., KHALIL S., SADDIQ B., AHMAD K., JAMIL Q.A., SHIRAZI J.H., HANIF M., UR-REHMAN M.S., KHAN K.U., and AZIZ M.I. Phytochemical, antimicrobial and time-kill kinetics potentials of Euphorbia nivulia Buch.-Ham.: A Cholistan desert medicinal plant. Pakistan Journal of Pharmaceutical Sciences, 2020, 33(5): 2461-2469.

AMRATI F.E., BOURHIA M., SAGHROUCHNI H., SLIGHOUA M., GRAFOV A., ULLAH R., EZZELDIN E., MOSTAFA G.A., BARI A., IBENMOUSSA S., and BOUSTA D. Caralluma europaea (Guss.) N.E.Br.: Anti-Inflammatory, Antifungal, and Antibacterial Activities against Nosocomial Antibiotic-Resistant Microbes of Chemically Characterized Fractions. Molecules, 2021, 26(3). DOI:

BALDEMIR A., KÖROĞLU A., ALTANLAR N., and COŞKUN M. A Comparative Study on the in vitro Antioxidant and Antimicrobial Potentials of Three Endemic Ononis L. Species from Turkey. Turkish Journal of Pharmaceutical Sciences, 2018, 15(2): 125-129. DOI:


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