Tender Coconut Water Сan Inhibit Inflammation Caused by Cigarette Smoke

Siti Thomas Zulaikhah, Sampurna, Joko Wahyu Wibowo, Helmia Fitri Nurul Aini, Arrizki Azka Pratama


Cigarette smoke contains numerous free radicals that can trigger an inflammatory response. The response stimulates the release of pro-inflammatory cytokines such as C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-8. Tender coconut water containing L-arginine has been shown to reduce inflammatory cytokines, including TNF-α, and IL-6. This study aims to determine the potential of tender coconut water in inhibiting inflammation in mice exposed to cigarette smoke. In this posttest only control group study, twenty-four male Wistar rats were randomly divided into 4 groups: K1 (standard ad libitum+ aqua diet); K2 (standard diet ad libitum + aqua + exposure to cigarette smoke 3 sticks/day); K3 (standard diet ad libitum + aqua+ exposure to cigarette smoke 3 sticks/day + vitamin E at a dose of 1.8 mg/200 grBW/day); K4 (standard diet ad libitum + aqua + exposure to cigarette smoke 3 sticks/day + tender coconut water at a dose of 8mL/200 grBW/day). The treatment was given for 14 days. On day 15, the blood samples were collected to evaluate CRP, IL-6, and TNF-α using ELISA (enzyme-linked immunosorbent assay). The data were analyzed using one way ANOVA test. The results showed that the level of CRP, IL-6m, and TNF-α in the group administrated with tender coconut water was significantly lower than that of those exposed to cigarette smoke alone (p<0.05). This study demonstrates that tender coconut water can inhibit inflammation in mice caused by cigarette smoke.


Keywords: tender coconut water, cigarette smoke, C-reactive protein, interleukin-6, tumor necrosis factor-alpha.

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