The Potential of Honey Supplementation in Extender for Preservation of Brek Fish (Systomus Orphoides) Spermatozoa
Abstract
The population of brek fish (Systomus orphoides) decreases due to exploitation and environmental changes. Therefore, efforts are being made to increase the population. However, these efforts face many obstacles, including the different maturation times of the male and female gonad anticipated by preserving the fish spermatozoa, where the success rate is affected by the extender used for spermatozoa dilution. Honey as an extender supplement is needed to protect and provide nutrition during preservation. This research aims to obtain the optimal concentration of honey as extender supplements for the spermatozoa after 48 hours of preserving break fish (S. orphoides). The concentrations of honey used were 0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The spermatozoa and eggs used were collected through stripping. The samples were stored in a refrigerator at 4°C for 48 hours. The post-preserved spermatozoa were evaluated by examining motility, viability, abnormalities, fertility, and hatchability. The data analysis was then conducted using a one-way ANOVA test and continued with Tukey's test. The results showed a significant difference (P<0.05) in the percentage of post-preserved spermatozoa motility, viability, abnormality, and eggs hatchability. The optimal concentration was discovered at 0.6% of honey, which produced the highest motility, viability, and eggs hatchability (82.35 ± 1.19%, 70.81 ± 1.06%, and 28.45 ± 6.27%, respectively). Besides, the lowest spermatozoa abnormality was 15.73 ± 0.62% after preservation. This study demonstrates for the first time optimized preservation protocol for S. Orphoides post-preserved sperm.
Keywords: eggs’ hatchability, brek fish (Systomus orphoides), spermatozoa quality, honey, preservation.
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