The Effects of Panoramic Radiography on Gingival Crevicular Fluid Volume and Micronucleus in Wistar Rats (Rattus Novergicus) with Periodontitis

Didit Aspriyanto, Bayu Indra Sukmana, Huldani, Aminuddin Prahatama Putra


Panoramic radiography is a radiographic diagnostic tool that affects the Reactive Oxygen Species and causes oxidative stress, which has a role in DNA damage that occurs due to periodontitis or panoramic radiographic radiation exposure is characterized by the formation of micronuclei in gingival epithelial cells. Panoramic radiography exposure and periodontitis can increase the Gingival Crevicular Fluid (GCF) flow because of increased vascular permeability. The present study aims to determine the effect of panoramic radiography on the volume of GCF and micronuclei in Wistar rats with periodontitis. This study was true experimental with post-test only and control group design, used 40 male Wistar rats. The average number of GCF in the healthy rats' group that was not exposed to panoramic radiography was 0.114 μL, the periodontitis rats group that was not exposed, once, twice, and three times exposed to panoramic radiography were 0.246, 0.286, 0.294, and 0.374 μL, respectively. The result of the One-Way Annova test and Post Hoc Bonferroni test indicated that significant changes in the number of micronuclei were seen between the normal group with once exposure to the periodontitis group with two times and three times exposure and in the periodontitis group without exposure to the periodontitis group with two times and three times exposure. Panoramic radiograph X-ray radiation causes an increase in the volume of GCF and changes the number of micronuclei in Wistar rats with periodontitis.


Keywords: panoramic radiography, Gingival Crevicular Fluid, micronucleus, periodontiti.

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PEREZ Z., GARCIA O., RAMOS L., VELAZQUEZ G., MEDA G., AGUILAR R., and GONZALEZ Z. Increased Micronuclei and Nuclear Abnormalities in Buccal Mucosa and Oxidative Damage in Saliva From Patient With Chronic and Aggressive Periodontal Diseases. Journal of Periodontal Research, 2016, 1(5).

EKAPUTRI S. & MASULILI S.L. Cairan Sulkus Gingiva Sebagai Indikator Keadaan Jaringan Periodontal. Majalah Kedokteran Gigi, 2016, 17(1): 75.

TADIN A., GAVIC L., ROGULJIC M., JERKOVIC D., and ZELJEZIC D. Nuclear Morphological Changes in Gingival Epithelial Cells of Patients With Periodontitis. Clinical Oral Investigations, 2019, 1(2).

SABERI B.V., NERMATI S., and JAVANMARD A. Assessment of Digital Panoramic Radiography’s Diagnostic Value in Angular Bony Lesions With 5 mm Or Deeper Pocket Depth In Mandibular Molars. Dental Research Journal, 2017; 14(1): 33.

SALMAN A. & MEETHIL A. An Unusual Presentation of Generalized Aggressive Periodontitis with Multiple Impacted Supernumerary Teeth. European Journal of Dentistry, 2017, 6.

THOMSON E.M. & JOHNSON O.N. Essentials of Dental Radiography for Dental Assistants and Hygienists. 9th ed. Pearson Education, USA, 2017: 378-380.

WHITE S.C. & PHAROAH M.J. Oral Radiology Principles and Interpretation.7th ed. Elsevier, Canada, 2013: 3-4; 16-17; 19.

NURIYAH L. & JUWONO A.M. Elektromagnetisme (Listrik-Magnet). UB Press, Malang, 2017. p.155.

ASRIWATI. Fisika Kesehatan Dalam Keperewatan. Penerbit Deepublish, Yogyakarta, 2017. p.167.

IANNUCCI J.M. & HOWERTON L.J. Dental Radiography Principles and Techniques. 5th ed. Elsevier, Canada, 2017: 12.

WHAITES E. & DRAGE N. Essentials of Dental Radiography and Radiology. 5th ed. Elsevier, China, 2017: 15; 65-66.

SHEIKH S., PALLAGATTI S., GREWEL H., KALUCHA A., and KAUR H. Genotoxicity of Digital Panoramic Radiography on Oral Epithelial Tissues. Quintessence International, 2018, 43(8): 719.

SAPUTRA D., ASTUTI E.R., DARANINGGAR A., ALAM A.R., SATRIA F.A., and AULIA M. The Effect Of Mangosteen Pericarp (Garcinia mangostana L.) Extract Mucoadhesive Gingival Patch On The Mda Levels And The Number Of Micronuclei Due To Panoramic Radiography Radiation. Indonesian Journal of Dental Medicine, 2019, 2(4): 508-514.

SETYAWATI H.A., DEWI N., and OKTAVIYANTI I.K. Analisis Sitogenik Mikronukleus Bukal Pada Orang Menginang dan Tidak Menginang. Dentino Jurnal Kedokteran Gigi, 2016, 1(1): 43.

RAMOS M.A., CURY F.D.P., NETO C.S., MARQUES M.M.C, and SILVEIRA H.C.S. Micronucleus Evaluation of Exfoliated Buccal Epithelial Cells Using Liquid-Based Cytology Preparation. Acta Cytologica, 2016: 582-584.

SHANTININGSIH R.R., SUWALDI, ASTUTI I., and MUDJOSEMEDI M. Peningkatan Jumlah Mikronukleus Pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik. Majalah Kedokteran Gigi, 2017, 20(2): 119-125.

RAHMAH N., DEWI N., and RAHARDJA S.D. Analisis Sitogenik Mikronukleus Mukosa Bukal Pada Perokok Aktif dan Pasif. Dentino Jurnal Kedokteran Gigi, 2016, 1(1): 16-19.

RUBIN P. & FAJARDO L. Biophysiopathology of the Microvasculature and Microcirculation. In: RUBIN P., CONSTINE L.S, MARKS L.B. (eds.) ALERT – Adverse Late Effects of Cancer Treatment Volume 1: General Concepts and Specific Precepts. Springer, London, 2018: 12; 30-31.

KURNIAWATI I., PUJIASTUTI P., and DHARMAYANTI A.W.S. Kadar Kalsium (Ca) dalam Cairan Krevikular Gingiva Pada Penderita Periodontitis Kronis. ODONTO Dental Journal, 2016, 2(2): 9-11.

KHARBANDA O.P. & SHARAN J. Periodontium. In: TALWAR GP, HASNAIN S.E., SARIN S.K. (eds.) Textbook of Biochemistry, Biotechnology, Allied and Molecular Medicine. PHI Learning Limited, New Delhi, 2016.

KHURSHID Z., MALI M., NASEEM M., NAJEEB S., and ZAFAR M.S. Human Gingival Crevicular Fluids (GCF) Proteomics: An Overview. Dentistry Journal. 2017; 5(12): 1-2.

KAYAR N.A. ODUNCUOGLU B.F. HALILOGLU S, SERPEK B, ATAOGLU T, and ALPTEKIN N.O. Methodological Evaluation of Gingival Crevicular Fluid Volume and Neutrophil Elastase Levels: Sequential Sampling, Length of Sampling Time and Two Different Sampling Methods. Acta Odontologica Scandinavica, 2019, 2.

NEWMAN M.G., TAKEI H.H., KLOKKEVOLD P.R., and CARRANZA F.A. Newman and Carranza’s Clinical Periodontology. 13th ed. Elsevier, Philadelphia, 2019: 27; 91; 96; 105; 237; 239.

REDDY S. Essentials of Clinical Periodontology and Periodontics. 5th ed. Jaypee Brothers Medical Publishers, New Delhi, 2018: 173-174.

ATTAR N.B., BANODKAR A.B., GAIKWAD R.P., SETHNA G.D., PATIL C.L., and SIMON S. Evaluation of gingival crevicular fluid volume in relation to clinical periodontal status with periotron 8000. International Journal of Applied Dental Sciences, 2018, 4(1): 70-71.

NOORMA, SUKMANA B.I., and SAPUTERA D. The Influence between the Length of Radiographer Working Time and the Reduction of Salivary pH (Research on Radiographers at RSUD Ulin Banjarmasin and RSGM Gusti Hasan Aman Banjarmasin in 2017). Dentino Jurnal Kedokteran Gigi, 2018, 3(1): 127.

BADAN PENGAWAS TENAGA NUKLIR. Pedoman Teknis Penyusunan Tingkat Panduan Diagnostik atau Diagnostic Reference Level. Nasional, 2019.

TSAPAKI V. Radiation Protection In Dental Radiology – Recent Advances and Future Directions. Physica Medica, 2017, 44: 224.

PRADEEP A.R., AGARWAL E., P. RAJU A., RAO M.S.N., and FAIZUDDIN M. Study of Orthophosphate, Pyrophosphate, Pyrophosphatase in Saliva with Reference to Calculus Formation and Inhibition. Periondotal Journal, 2016, 82(3): 445.

SUSANTININGSIH T. Obesitas dan Stres Oksidatif. JuKe Unila, 2017, 5(9): 90.

WANG Y., ANDRUKHOV O., and FAN X.R. Oxidative Stress and Antioxidant System in Periodontitis. Frontiers in Physiology, 2017, 8.

PEREZ Z., GONZALEZ G., RAMOS L., VELAZQUEZ G., and PARADA S. Periodontal Disease and Nuclear and Oxidative DNA Damage. Insights into Various Aspects of Oral Health, 2017: 29-30.

FENECH M., KIRSCH-VOLDERS M., NATARAJAN A.T., SURRALLES J., CROTT J.W, and PARRY J. Molecular Mechanism of Micronucleus, Nucleoplasmic Bridge and Nuclear Bud Formation In Mammalian And Human Cells. Mutagenesis, 2017, 26(1): 126.

BERNIYANTI T. Biomarker Toksisitas Paparan Logam Tingkat Molekuler. Pusat Penerbitan dan Percetakan Universitas Airlangga, Surabaya, 2018: 60.

BASTOS-AIRES D., ALVARO A., MARA L.P., DANIEL P.M., and ALEXANDRA T. Preliminary Study of Micronuclei Levels in Oral Exfoliated Cells From Patients With Periodontitis. Journal of Dental Sciences, 2018, 8: 200-204.

CHATTERJEE E.M.M. & MEIRELES J.R.C. The Use of the Micronucleus Test to Monitor Individuals at Risk of Oral Cancer. The Research and Biology of Cancer, 2016, 2-3.

ZUELKEVIN. Effects of panoramic radiographic exposure to gingival sulcus (GCF) fluid volume. Thesis. Faculty of Dentistry, Universitas of Gadjah Mada, Bahasa Indonesia, 2015.


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