Immune Response (Cortisol, TNFΑ, HMGB1) in Trained and Untrained Adolescent after 12 Minutes Run Exercise

Ilhamjaya Pattelongi, Huldani, Muhammad Nasrum Massi, Irfan Idris


This study aims to explain the immune response (cortisol, TNF Alpha, HMGB1) after 12 minutes of moderate-intensity aerobic exercise. This exercise is a modification of Cooper’s 12-minute running test that can be done by running 20 meters for 12 minutes according to an individual’s ability with moderate intensity 60-80% maximum pulse rate. The research was conducted on 15 trained and 15 untrained students at SMAN 1 Banjarbaru. The sampling technique was the purposive sampling method. The value of VO2 max was measured on the first day with Multistage Fitness Test. Cortisol, TNF Alfa, HMGB1 was measured by taking blood test. Data analysis used the Mann-Whitney test. The results showed that the average VO2 max, cortisol, and HMGB1 scores of students with basketball achievement were higher than those of untrained students. TNF alpha level that did not increase was higher. The data analysis showed the difference in measurements between trained and untrained students (p < 0.05) on VO2 max, Cortisol, and TNF Alpha. There was no significant difference in HMGB1 (p > 0.05). There is a positive correlation between Cortisol and TNF Alpha, Cortisol and HMGB1, HMGB1 and TNF Alpha in adolescents. It can be concluded that trained adolescents’ Cortisol levels increased higher. Trained adolescents’ TNF alpha levels were lower. It was not proven that trained students’ HMGB1 levels differed from those of untrained ones. In adolescents, there is an increase in cortisol levels followed by an increase in TNF Alpha levels, an increase in cortisol levels followed by an increase in HMGB1 levels, and an increase in HMGB1 levels followed by an increase in TNF Alpha levels. Moderate-intensity aerobic exercise (12 minutes of running) can be recommended as simple exercises to maintain fitness and boost the immune system or exercise health during a pandemic.


Keywords: immune response, 12 minutes of running, trained adolescents, untrained adolescents.

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BENNIE JA, DE COCKER K, TEYCHENNE MJ, et al. The epidemiology of aerobic physical activity and muscle-strengthening activity guideline adherence among 383,928 U.S. adults. International Journal of Behavioral Nutrition and Physical Activity, 2019, 16(1): 1-11.

SINGH R, PATTISAPU A, and EMERY MS. US Physical Activity Guidelines: Current state, impact and future directions. Trends in Cardiovascular Medicine, 2019, 30(7): 407-412.

MALLO FERRER F. Physical Activity and Exercise. In HUHTANIEMI I, and MARTINI L. (Eds) Encyclopedia of Endocrine Diseases, 2nd ed. Academic Press; 2018, 436–441.

PIEPOLI MF, HOES AW, AGEWALL S, et al. European Guidelines on cardiovascular disease prevention in clinical practice. European Heart Journal, 2016, 37(29): 2315–2381.

PATEL H, ALKHAWAM H, MADANIEH R, et al. Aerobic vs anaerobic exercise training effects on the cardiovascular system. World Journal of Cardiology, 2017; 9(2): 134.

MULYONO M. Pengaruh Manipulasi Sport Massage Terhadap Penurunan Denyut Nadi Setelah Latihan Olahraga. Jurnal Sportif, 2016, 2(2): 15-21.

FAN W and EVANS RM. Exercise mimetics: impact on health and performance. Cell Metabolism, 2017, 25(2): 242-247.‏

KJØBSTED R, HINGST JR, FENTZ J, et al. AMPK in skeletal muscle function and metabolism. The FASEB Journal, 2018, 32(4): 1741-1777.‏

LEE DC, PATE RR, LAVIE CJ, et al. Leisure-time running reduces all-cause and cardiovascular mortality risk. Journal of the American College of Cardiology, 2014, 64(5): 472–481.

KOHRT WN, MALLEY MT, COGGAN AR, et al. Effects of gender, age, and fitness level on response of iio, max to training in 60-71 yr olds. Journal of Applied Physiology, 1991, 70(5): 2004-2011.

CARAZO-VARGAS P. and MONCADA-JIMÉNEZ J. A meta-analysis on the effects of exercise training on the VO2max in children and adolescents. Retos, 2015, 27: 184-187.

BAQUET, G., BERTHOIN, S., DUPONT, G., et al. Effects of high intensity intermittent training on peak VO(2) in prepubertal children. International Journal of Sports Medicine, 2002, 23(6): 439- 444.

HUANG G, GIBSON CA, TRAN ZV, OSNESS WH. Controlled Endurance Exercise Training and VO2max Changes in Older Adults: A Meta-Analysis, Preventive Cardiology, 2005, 8(4): 217-225.

BUDDE H, MACHADO S, RIBEIRO P, and WEGNER M. The cortisol response to exercise in young adults. Frontiers in Behavioral Neuroscience, 2015, 9, article ID 13.

DUCLOS M, and TABARIN A. Exercise and the Hypothalamo-Pituitary-Adrenal Axis. Frontiers of Hormone Research, 2016, 47: 12-26.

HASLINDA DS, PATELLONGI I, and SINRANG AW. Pengaruh Olahraga Futsal Terhadap Kadar Kortisol Serum Pada Individu Dewasa Muda. In Seminar Nasional LP2M UNM. 2017, 2(1).

SATO K, IEMITSU M, KATAYAMA K, et al. Responses of sex steroid hormones to different intensities of exercise in endurance athletes. Experimental Physiology, 2016, 101(1): 168-175.

MUSCELLA A, VETRUGNO C, SPEDICATO M, et al. The effects of training on hormonal concentrations in young soccer players. Journal of Cellular Physiology, 2019, 234(11): 20685-20693.

INCE LM, WEBER J, and SCHEIERMANN C. Control of leukocyte trafficking by stress-associated hormones. Frontiers in Immunology, 2019, 10(JAN): 1-9.

BESERRA AHN, KAMEDA P, DESLANDES AC, et al. Can physical exercise modulate cortisol level in subjects with depression? A systematic review and meta-analysis. Trends in Psychiatry and Psychotherapy, 2018, 40(4): 360-368.

WANG CC, ALDERMAN B, WU CH, et al. Effects of Acute Aerobic and Resistance Exercise on Cognitive Function and Salivary Cortisol Responses. Journal of Sport & Exercise Psychology, 2019; 41(2):73-81.

DIMITROV S, HULTENG E., & HONG S. Inflammation and exercise: Inhibition of monocytic intracellular TNF production by acute exercise via β2-adrenergic activation. Brain, Behavior, and Immunity, 2017, 61: 60–68.

TERINK R, BONGERS CCWG, WITKAMP RF, et al. Changes in cytokine levels after prolonged and repeated moderate intensity exercise in middle-aged men and women. Translational Sports Medicine, 2018, 1(3): 110–119,

NEMET D, OH Y, KIM HS, HILL M, and COOPER DM. Effect of intense exercise on inflammatory cytokines and growth mediators in adolescent boys. Pediatrics, 2002, 110(4): 681-689.

STEENSBERG A, KELLER C, STARKIE R L, et al. IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle. American Journal of Physiology, Endocrinology and Metabolism, 2002, 283(6): E1272–E1278.

HULDANI, ACHMAD H, ARSYAD A, et al. Differences in VO2 max based on age, gender, hemoglobin levels, and leukocyte counts in Hajj prospective pilgrims in Hulu Sungai Tengah Regency, South Kalimantan. Systematic Reviews in Pharmacy, 2020, 11(4): 9–14.

SOUGLIS A, BOGDANIS GC, GIANNOPOULOU I, et al. Comparison of Inflammatory Responses and Muscle Damage Indices Following a Soccer, Basketball, Volleyball and Handball Game at an Elite Competitive Level. Research in Sports Medicine, 2015, 23(1): 59–72.

HULDANI. Pengaruh Latihan Aerobik Ringan dan Sedang terhadap Kadar Interleukin 8 dan Jumlah Netrofil pada Remaja. Berkala Kedokteran. 2016, 12(1):61-7.‏

VOURC’H M, ROQUILLY A, and ASEHNOUNE K. Trauma-Induced Damage-Associated Molecular Patterns-Mediated Remote Organ Injury and Immunosuppression in the Acutely Ill Patient. Frontiers in Immunology, 2018; 9: article ID 1330,

YANG H, WANG H, CHAVAN SS, and ANDERSSON U. High Mobility Group Box Protein 1 (HMGB1): The Prototypical Endogenous Danger Molecule. Molecular Medicine, 21 (SUPPLEMENT 1), 2015, S6-S12.

GOH J, HOFMANN P, AW NH, et al. Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory biomarkers in healthy young men: a pilot study. Translational Medicine Communications, 2020, 5(1),

KAKI A, NIKBAKHT M, HABIBI A, and FATHI MOGHADAM H. The effect of aerobic exercise on HMGB1 protein levels and some oxidative stress indices in rats with diabetic neuropathic pain. Daneshvar Medicine: Basic and Clinical Research Journal, 2020; 27(3): 27-38, 10.22070/dmed.27.142.27

BEKOS CM, ZIMMERMANN L, UNGER S, et al., Non-professional marathon running: Rage axis and st2 family changes in relation to open-window effect, inflammation and renal function, Scientific Reports, 2016, 6: 32315.

BEHRINGER M, KILIAN Y, MONTAG J, et al. Plasma concentration of high-mobility group box 1 (HMGB1) after 100 drop to vertical jumps and after a 1200-km bicycle race. Research in Sports Medicine, 2016, 24(2): 119-129,

GMIAT A, MIESZKOWSKI J, PRUSIK K, et al., Changes in pro-inflammatory markers and leucine concentrations in response to nordic walking training combined with vitamin d supplementation in elderly women. Biogerontology, 2017, 18: 535-548.


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