Immune Response (Cortisol, TNFΑ, HMGB1) in Trained and Untrained Adolescent after 12 Minutes Run Exercise

Ilhamjaya Pattelongi, Huldani, Muhammad Nasrum Massi, Irfan Idris

Abstract

This study aims to explain the immune response (cortisol, TNF Alpha, HMGB1) after 12 minutes of moderate-intensity aerobic exercise. This exercise is a modification of Cooper’s 12-minute running test that can be done by running 20 meters for 12 minutes according to an individual’s ability with moderate intensity 60-80% maximum pulse rate. The research was conducted on 15 trained and 15 untrained students at SMAN 1 Banjarbaru. The sampling technique was the purposive sampling method. The value of VO2 max was measured on the first day with Multistage Fitness Test. Cortisol, TNF Alfa, HMGB1 was measured by taking blood test. Data analysis used the Mann-Whitney test. The results showed that the average VO2 max, cortisol, and HMGB1 scores of students with basketball achievement were higher than those of untrained students. TNF alpha level that did not increase was higher. The data analysis showed the difference in measurements between trained and untrained students (p < 0.05) on VO2 max, Cortisol, and TNF Alpha. There was no significant difference in HMGB1 (p > 0.05). There is a positive correlation between Cortisol and TNF Alpha, Cortisol and HMGB1, HMGB1 and TNF Alpha in adolescents. It can be concluded that trained adolescents’ Cortisol levels increased higher. Trained adolescents’ TNF alpha levels were lower. It was not proven that trained students’ HMGB1 levels differed from those of untrained ones. In adolescents, there is an increase in cortisol levels followed by an increase in TNF Alpha levels, an increase in cortisol levels followed by an increase in HMGB1 levels, and an increase in HMGB1 levels followed by an increase in TNF Alpha levels. Moderate-intensity aerobic exercise (12 minutes of running) can be recommended as simple exercises to maintain fitness and boost the immune system or exercise health during a pandemic.

 

Keywords: immune response, 12 minutes of running, trained adolescents, untrained adolescents.


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