Spectrophotometric Determination of Salbutamol and Meptazinol in Drug Formulations Using the Berthelot Reaction

Ekhlas Qanber Jasim, Munther Abduljaleel Muhammad-Ali, Hawraa K. Dhaef, Maged H. Al-Safee


A rapid and delicate spectrophotometric tactic was developed and associated with drug betrothal in pharmaceutical definitions for the audacity of a few phenolic enclosures (salbutamol or meptazinol). The way is based on oxidative coupling revulsion of these compromise with sulfapyridine and ethyl p-aminobenzoate in the person of potassium meta periodate as an oxidizing agent. The formed blue indophenol dyes have maximum absorptions at 670, 690, 630, and 650 nm for salbutamol with benzocaine (D1), salbutamol with sulfapyridine (D2), meptazinol with benzocaine (D3) and, meptazinol with sulfapyridine (D4), respectively. The spectrophotometric determination of these phenolic compounds using molar absorptivity are 2.969× 103, 5.848× 103, 2.724 × 103, and 2.172 x 103 L mol-1. cm-1 for concentrations obeyed Beer's law in the ranges 2-20, 1-16, 2-14, and 1-12 µg ml-1 for the above compounds, respectively. The average recovery % was ranged between (99.45% - 100.51%) with a relative standard deviation ≤ 0.111 for all the studied compounds. The method is applied successfully to the assay of salbutamol and meptazinol.

Keywords: indophenols, phenolic compounds, Job’s method, Beer’s law, Berthelot reaction.

Full Text:



FREI A. Metal Complexes, an Untapped Source of Antibiotic Potential. Antibiotics, 2020, 9(2): 1-24. https://doi.org/10.3390/antibiotics9020090

GRGI J., ŠELO G., PLANINI' M., TIŠMA M., and BUCI'C-KOJI'C A. Role of the Encapsulation in Bioavailability of Phenolic Compounds. Antioxidants, 2020, 9(10): 1-35. https://doi.org/10.3390/antiox9100923

HAPSARI B. W., MANIKHARDA, and SETYANINGSIH W. Methodologies in the Analysis of Phenolic Compounds in Roselle (Hibiscus sabdariffa L.): Composition, Biological Activity, and Beneficial Effects on Human Health. Horticulturae, 2021, 7(2): 1-41. https://doi.org/10.3390/horticulturae7020035

GARDINER A. M., & WILKINSON H. M. Randomized Clinical Trial Comparing Breath-Enhanced to Conventional Nebulizers in the Treatment of Children with Acute Asthma. The Journal of Pediatrics, 2019, 204: 245-249. https://doi.org/10.1016/j.jpeds.2018.08.083

SUAU J. S., & DEBLIEUX M. C. P. Management of Acute Exacerbation of Asthma and Chronic Obstructive Pulmonary Disease in the Emergency Department. Emergency Medicine Clinics of North America, 2016, 34: 15–37. https://doi.org/10.1016/j.emc.2015.08.002

SINGER J., JANK A., AMARA S., STEPAN P. D. H., KAISERS U., and HOEHNE C. Efficacy and Effects of Parenteral Pethidine or Meptazinol and Regional Analgesia for Pain Relief during Delivery. A Comparative Observational Study. Geburtshilfe Frauenheilkd, 2016, 76(9): 964–971. https://doi.org/10.1055/s-0042-111009

SPINELLI B. J., KELLEY P. L., and HAIGIS C. M. An LC-MS Approach to Quantitative Measurement of Ammonia Isotopologues. Scientific Reports, 2017, 7: 1-8. https://doi.org/10.1038/s41598-017-09993-6

HADI H. Simultaneous spectrophotometric determination of vitamin B6 by coupling with p-amino phenol. International Journal of Pharmaceutical Quality Assurance, 2016, 7(4): 84-89. https://doi.org/10.25258/ijpqa

ALODAINI H. A., ALOTAIBI N. T., AHMAD D., OMER M. E., VASUDEVAN S., and MANSOUR M. Stability Study of Valsartan in Aqueous Solutions: Effect of different pH, Time and Temperature. International Journal of Research in Pharmacy and Science, 2019, 8(3): 1-15. https://www.researchgate.net/publication/336832087_Stability_study_of_Valsartan_in_Aqueous_Solutions_Effect_of_different_pH_Time_and_Temperature#:~:text=The%20present%20study%20showed%20that,can%20significantly%20lower%20valsartan%20concentrations.

HUMEDY I. T., & ALI I. M. Spectrophotometric Determination of Sulfathiazole Using 2,4–dinitrophenylhydrazine as Coupling Reagent. Ibn Al-Haitham Journal for Pure & Applied Science, 2016, 29(2): 227-239. https://en.uobaghdad.edu.iq/jih/index.php/j/article/view/112

LIMPANUPARB T., ROONGRUANGSREE P., and AREEKUL C. A DFT investigation of the blue bottle experiment: E◦ half-cell analysis of autoxidation catalyzed by redox indicators. Royal Society Open Science, 2017, 4: 1-14. http://dx.doi.org/10.1098/rsos.170708

MULLER J. D., SCHNEIDER B., AßMANN S., and REHDER G. Spectrophotometric pH measurements in the presence of dissolved organic matter and hydrogen sulphide. Limnol. Oceanography: Methods, 2018, 16: 68–82. https://doi.org/10.1002/lom3.10227

AL–RUFAIE M. M. Spectrophotometric determination of sodium salicylate in pharmaceutical preparations by coupling with diazotized para-aminobenzoic acid. Bioequivalence & Bioavailability, 2016, 2(2): 17‒2. https://doi.org/10.15406/mojbb.2016.02.00020

R.SIDDIQUI M., AL-OTHMAN Z. A., and RAHMAN N. Analytical techniques in pharmaceutical analysis: A review. Arabian Journal of Chemistry, 2017, 10: 1409–1421. https://doi.org/10.1016/j.arabjc.2013.04.016


  • There are currently no refbacks.