Effect of the Methanol Extract of Centella Asiatica as a Chemotherapeutic Agent on the Occurrence and Development of Dysplastic Cells: Histological and Immunohistochemical Analysis via the p53 and tnf-α Genes

Ahyar Riza, Gostry Aldica Dohude, Jovanna Clarissa Hutapea, Nur Farah Nabilah

Abstract

This research evaluated the efficacy of Centella asiatica methanol extract at various concentrations on the emergence and progression of dysplastic cells induced by dimethylbenz(a)anthracene (DMBA) in male Wistar rats. The study aimed to demonstrate that the methanol extract of Centella asiatica is a phytochemically effective and histologically and immunohistochemically proven inhibitor of oral dysplasia cell development. Fifty male Wistar rats (Rattus novergicus) were divided into five groups, with four groups receiving varying doses of the treatment and one group serving as a positive control. Phytochemical screening was conducted using qualitative and quantitative methods. Histopathological examination was performed using H&E and IHC staining techniques. Qualitative screening revealed alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoid/steroid compounds in the methanol extract of Centella asiatica. Quantitative screening revealed the levels of total phenolics, total flavonoids, and antioxidant activity. The study demonstrated the effectiveness of the methanol extract of Centella asiatica at different concentrations in reducing the incidence of DMBA-induced dysplasia. The groups not administered the extract exhibited higher dysplasia grades, indicating the efficacy of administering the methanol extract of Centella asiatica at varying doses in reducing DMBA-induced dysplasia.

 

Keywords: methanol extract of Centella asiatica, DMBA, dysplasia.

 

https://doi.org/10.55463/issn.1674-2974.51.6.26


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