Optimization and Validation of Spectrophotometric Methods for the Determination of Prednisolone in Chitosan Nanoparticles
Abstract
This study validated a UV spectrophotometric method for quantifying prednisolone in pharmaceutical formulations. The method was selective, linear, accurate, precise, and compatible with filtration. The determined linearity range 0.6-25 µg/mL was based on a correlation value of 0.9996. Accuracy was determined through recovery trials, confirming recovery within 98.5%–101.5% of the initial value, with %RSD of less than 2.0 for all levels. Precision was meticulously evaluated through intra-day and inter-day trials, revealing a relative standard deviation (RSD) of less than 2%, attesting to the method’s remarkable accuracy. In addition, the study also evaluated the wavelength of maximum absorption for prednisolone (246 nm) and the method's selectivity against commonly used excipients in pharmaceutical formulations. The method was also stable under different conditions, including temperature, pH, and light. This study demonstrated that the UV spectrophotometric method is reliable and robust for quantifying prednisolone in pharmaceutical formulations. It is simple, rapid, and cost-effective, making it a valuable tool for quality control and research. This study successfully achieved its stated goal of developing a simple, rapid, and cost-effective UV spectrophotometric method for quantifying prednisolone in pharmaceutical formulations with good selectivity, linearity, accuracy, and precision. This offers advantages over the standard pharmacopeial high-performance liquid chromatography (HPLC) method in being a simpler, faster, and more cost-effective analytical method while retaining satisfactory accuracy and precision.
Keywords: ultraviolet spectrophotometry, prednisolone quantification, drug, method validation.
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