Effect of Black Crab (Scylla Serrata) Chitosan Gel on the Three-Dimensional Socket Response and Fibroblasts after Tooth Extraction in Rattus Norvegicus

Hendry Rusdy, Gostry Aldica Dohude, Bagoes Anggoro Cesar Putro, Nasywa Tiara Syadana, Samuel Kevin


Tooth extraction is a common procedure in dentistry that can result in wounds to the soft and hard tissues of the alveolar process, thereby triggering the healing process. The wound healing process is often complicated by several factors, leading to delayed wound healing. The principles of wound healing involve four phases: hemostasis, inflammation, proliferation, and remodeling. This study aimed to determine the effect of black crab (Scylla serrata) chitosan gel on three-dimensional socket response and fibroblast proliferation after tooth extraction in Wistar rats (Rattus norvegicus). In this study, chitosan material with a degree of deacetylation of 84.9% was used, thus exhibiting good biological properties in the wound healing process. This research is an experimental study with a post-test only with a controlled group design. The sampling technique used was purposive sampling, and the sample size was calculated using the Federer formula. Observations were made by measuring the mesial-distal, lingual-buccal, and socket depths using calipers and the UNC15 probe and observing the number of fibroblasts histologically on Days 1, 3, and 7. The results of the one-way ANOVA and post hoc LSD tests indicate significant outcomes in socket wound closure and fibroblast proliferation in the chitosan gel group. This study demonstrates that black crab (Scylla serrata) chitosan gel is effective in accelerating socket wound closure and stimulating fibroblasts in socket wounds after tooth extraction.


Keywords: tooth extraction, black crab chitosan gel, socket response, fibroblast.



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