Evaluation of Chenopodium Album Extracts as Antimalarial Agents Using MTT Assay
Abstract
This article describes testing the antiplasmodial activity of Chenopodium album plant extracts against P. falciparum isolates using a tetrazolium (MTT) colorimetric assay. This idea is based on the need to develop new antimalarial drugs due to the high resistance of malaria parasites to available drugs. Therefore, we tested extracts of Chenopodium album against P. falciparum isolates for their antiplasmodial activity in the Mardan district. Fresh blood samples from P. falciparum infected patients were tested successfully using a tetrazolium (MTT) colorimetric assay for activity of Chenopodium album plant extracts in different solvents including n-Hexane, chloroform, acetone, ethanol, methanol and distilled water. 50% inhibitory concentrations (IC50) were noted 9.961, 5.387, 7.82, 7.9, 4.017, 0.524 μg/mL in these solvents. Our results indicate that all extracts tested inhibited the cultured parasites in concentrations ranging from 0.524 to 9.961 μg/mL, of which the aqueous extract being the most active showed excellent antiplasmodial activity (IC50 = 0.524 μg/mL). The results of this study develop and supplement the search for new antimalarial drugs from natural sources and the MTT assay can be used as a diagnostic test. The novelty of the results lies in the significant antiplasmodial activity of C. album extracts against P. falciparum isolates.
Keywords: plant extract, Chenopodium album, P. falciparum.
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