Testing the Toxicity, Protein Content, and Anticholesterol of the Ethanol Extract of the Sea Urchin (Diadema Setosum) Gonad as Marine Biodiversity-Based Medicinal Ingredients

Yusuf Sabilu, Jafriati, Asnia Zainuddin


Indonesia has various marine resources that can be used, one of which is a sea urchin (Diadema setosum), which can be used as pharmacology and food sources by using their gonad. This study aims to test the toxicity, protein content, and antioxidant activity of the ethanol extract of the sea urchin gonad. The sample tests consisted of several stages, namely: maceration performed the extraction using 96% ethanol solvent; the toxicity test used the Brine Shrimp Lethality Test method with Artemia salina Leach as the bioindicator; the protein content test used the Lowry method; anticholesterol activity test was done with ultraviolet-visible spectrophotometry (UV-Vis) method. The results showed that the ethanol extract of the sea urchin gonad had no toxic activity because the IC50 value was more than 1000 ppm, which was 1318.8639 ppm. The protein content of the ethanol extract of the sea urchin gonad was 0.0257% and had no anticholesterol potential with a value of 0.0002%. This research can be performed by conducting tests to determine aspects of reproductive biology and the anti-inflammatory effects of Diadema setosum.


Keywords: Diadema setosum, toxicity, protein content, anticholesterol.




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ZAKARIA I.J. Community of sea urchins (Echonoidea) on Cingkuak Island, Sikuai Island and Satan Island, West Sumatra. In: Proceeding of SEMIRATA International Conference on Science and Technology, 10-12 May 2013. 2013, 1(1): 381-387.

NANE L. Sustainability Study of Sea Urchin Fisheries Based on Biological, Ecological and Technological Dimensions Around Tolandono Island and Pulausawa Wakatobi Conservation Area. Thesis. Hasanuddin University, 2019. https://Marxiv.Org/9zdvr/

NANE L. Utilization of Sea Urchin Eggs Diadema setosum on Taliabu Island, North Maluku, Indonesia. 2020. https://doi.org/10.31219/osf.io/kmtuv

NANE L., and PARAMATA A.R. (2020). Impact of Overfishing on Density and Test-Diameter Size of the Sea Urchin Tripneustes gratilla at Wakatobi Archipelago, South-Eastern Sulawesi, Indonesia. Indonesian Journal of Marine Sciences, 25(2): 53–56.

https://doi.org/10.14710/ik.ijms.25. 2.53-56

NANE L. Sapurata Technology Machine Efficiency in Optimizing the Production of Kukure Food Innovations on Barrang Lompo Island, Makassar. 2019. https://osf.io/preprints/marxiv/q8spg/

LESTIONO L., and KRESNAMURTI A. (2020). Analgesic activity of ethanol extract of sea urchins (Echinometra mathaei) on male white mice. Journal of Herbs, Clinical and Pharmaceutical Science, 1(2): 7-12. http://dx.doi.org/10.30587/herclips.v1i02.1352

IRIANTO A., JAHIDIN, and SUDARAJAT H.W. The abundance of sea urchins (Echinoidea) in the intertidal waters of Liwutongki Island in Siompu District, South Buton Regency. Amphibious Journal, 2016, 1(2): 27-30.

SUSENO S. H., and JACOEB A.M. Fatty Acid and Amino Acid Profile of Sea urchins. Indonesian Journal of Fishery Products Processing, 2014, 17(1).

EVI M., ALIMUDDIN A.H., and DESTIARTI L. Utilization of extract of sea urchin (Diadema setosum) from Lemukutan Island as antifungal (Candida albicans). Chemical Kartika Journal, 2015, 4(4): 61-65.

BINTANG M. Biochemistry: Research Techniques. 2nd ed. Erlangga, Jakarta, 2018.

MEYER B.N., FERRIGNI N.R., PUTNAM J.E., JACOBSEN L.B., NICHOLS D.E., and MCLAUGHLIN J.L. Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Journal of Medicinal Plants Research, 1982, 45(5): 31-34.

LOWRY H.O., ROSENBROUGH N.J., FARR A.L., and RANDALL R.J. Protein Measurement with the Folin Phenol Reagent. Journal of Biology and Chemistry, 1951, 193: 265-275.

AMIN M.S. In-vitro Studies: Anticholesterol Effect of Methanol Extract of Parijoto Fruit (Medinilla speciosa Blume) Against Total Cholesterol. Thesis. Syarif Hidayatullah State Islamic University, Jakarta, 2015.

CARBALLO J.L., HERMANDES Z.L., PEREZ P., and GARCIA M.D. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnology, 2002, 2: 1-5.

PUTRI M.K.D., PRINGGENIES D., and RADJASA O.K. Phytochemical and Toxicity Test of Gastropod Crude Extract (Telescopium telescopium) Against Artemia salina Larvae. Journal of Marine Research, 2012, 1(2): 58-66.

SABILU Y., and JAFRIATI R.M. Test of bioactitvity and antioxidant activity of sea urchin (diadema setosum) gonads as medicinal ingredients based on marine biodiversity. Journal of Southwest Jiaotong University, 2022, 57(1).

APRILIA H.A., PRINGGENIES D., and YUDIATI E. Toxicity test of chloroform extract of sea urchin (Diadema setosum) shell and spines on mortality of nauplius (Artemia sp). Journal of Marine Research, 2012, 1(1): 75-83. https://doi.org/10.14710/jmr.v1i1.890

DAHL W.J., JEBSON P., and LOUIS D.S. Sea urchin injuries to the hand: A case report and review of the literature. The Iowa Orthopaedic Journal, 2010, 30: 153-156.

ABUBAKAR L., WANGI C., UKU J., and NDIRANGU S. Antimicrobial activity of various extracts of the sea urchin Tripneustes gratilla (Echinoidea). African Journal of Pharmacology and Therapeutics, 2012, 1(1): 19-23.

MCALISTER J.S., and MORAN A.L. Relationships among Egg Size, Composition, and Energy: A Comparative Study of Geminate Sea Urchins. PLoS ONE, 2012, 7(7): e41599. https://doi.org/10.1371/journal.pone.0041599

TUPAN J., and BR SILABAN B. Physical-Chemical Characteristics of Diadema setosum sea urchins from several Ambon Island waters. Triton , 2017, 13(2): 71-78.

ANGGRAINI D.I., and NABILLAH L.F. Activity test of suji leaf extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering. Journal of Chemical Research and Application, 2018, 21(2): 54-58.


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