Quantification of Triterpene Saponins of Hedera Helix Spray-Dried Extract by HPLC

Sidra Yasmeen, Sadaf Yasmeen, Saif Ur Rehman Khattak, Safila Navead, Yusra Almas, Najam Us Saquib, Halima Sadia, Fatima Qamar, Aisha Sana, Tajala Aman, Mohammad Ulusyar Khan

Abstract

Traditionally, ivy leaf (Hedera helix) has been used for a large number of ailments. In the 19th century, water-based extract of young leaves was used for respiratory diseases. The aim of the current study was to quantify the triterpene saponin named hederacoside C of ivy leaf spray-dried extract of Hedera helix using high-performance liquid chromatography (HPLC) method coupled with UV-visible detector. The solvent system consisted of solvent A (phosphoric Acid 85%: acetonitrile: water with the ratio of 2: 140: 860) and solvent B (phosphoric acid 85%: acetonitrile with the ratio of 2:998). A gradient elution was used for separation at 205 nm at a flow rate of 1.5 mL/min. The separation was performed using a C18 column at a temperature of 40°C. The method was validated as per ICH guidelines for precision, accuracy, recovery, ruggedness, and robustness. The method was found to be precise, accurate, robust, and reproducible according to the guidelines of United States Pharmacopeia 2014 and European Pharmacopeia 8.0. The content of triterpene saponins was found to be 17.6%. Despite many studies reported on the method development and quantification of compounds of Hedera helix, there is insufficient work reported on the spray-dried extracts of this plant. This study quantifies the hederacoside C from the spray-dried extract of the plant by developing an accurate, cheap, robust, and precise method. The proposed method can be of significant usage in the pharmaceutical industry.

 

Keywords: Hedera helix, spray drying, triterpene saponins, hederacoside C, high-performance liquid chromatography.

 

https://doi.org/10.55463/issn.1674-2974.49.9.3


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References


BOZUKOV V. Family Araliaceae in the Bulgarian macrofossil flora. Phytologia Balcanica, 2018, 24(1): 3-10. http://www.bio.bas.bg/~phytolbalcan/PDF/24_1/PhytolBalcan_24-1_01_Bozukov.pdf

BEKAR M., & GÜNEROĞLU N. Utilization of Living Walls in Urban Ecosystems. In: EFE R., CÜREBAL İ., GAF A., and TOTH B. (eds.) Enviromenthal Sustainability and Landscape Management. St. Kliment Ohridski University Press, Sofia, 2016: 467-482.

OKERMAN A. Combating the “Ivy Desert”: The Invasion of Hedera helix (English Ivy) in the Pacific Northwest United States. Restoration and Reclamation Review, 2000, 6(4): 1-10. https://conservancy.umn.edu/bitstream/handle/11299/59738/1/6.4.Okerman.pdf

FRIDLENDER A., & PECH N. About a tetraploid ivy in Sicily: from autochthonous Hedera to horticultural-invasive-hybrid package?, 2019. https://www.biorxiv.org/content/10.1101/751743v1

YOUNIS A., RIAZ A., SABI-UL-HASAN N. M., and HAMEED M. Response of Hedera helix (English Ivy) to various salinity levels. Journal of Horticultural Science and Biotechnology, 2014, 18: 1-6.

DEMIRCI B., GOPPEL M., DEMIRCI F., and FRANZ G. HPLC profiling and quantification of active principles in leaves of Hedera helix L. Die Pharmazie - An International Journal of Pharmaceutical Sciences, 2004, 59(10): 770-774. https://www.ingentaconnect.com/content/govi/pharmaz/2004/00000059/00000010/art00006

STAUSS-GRABO M., ATIYE S., WARNKE A., WEDEMEYER R., DONATH F., and BLUME H. Observational study on the tolerability and safety of film-coated tablets containing ivy extract (Prospan® Cough Tablets) in the treatment of colds accompanied by coughing. Phytomedicine, 2011, 18(6): 433-436. https://doi.org/10.1016/j.phymed.2010.11.009

DHAWAN K., KUMAR S., and SHARMA A. (eds.) Passiflora incarnata Linn.: a promising anxiolytic and sedative-isolation of a benzoflavone moiety as the bioactive agent. Presentation at the 42nd Annual Meeting of the American Society of Pharmacognosy, 2001.

BLUMENTHAL M. Therapeutic guide to herbal medicines. American Botanical Council, Austin, Texas, 1998.

OZDEMIR C., SCHNEIDER L. A., HINRICHS R., STAIB G., WEBER L., WEISS J. M., and SCHARFFETTER-KOCHANEK K. Allergic contact dermatitis to common ivy (Hedera helix L.). Der Hautarzt, 2003, 54(10): 966-969. https://doi.org/10.1007/s00105-003-0584-4

REHMAN S. U., CHOI M. S., KIM I. S., KIM S. H., and YOO H. H. An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma. Journal of Pharmaceutical and Biomedical Analysis, 2016, 129: 90-95. https://doi.org/10.1016/j.jpba.2016.06.039

YU M., SHIN Y. J., KIM N., YOO G., PARK S., and KIM S. H. Determination of saponins and flavonoids in ivy leaf extracts using HPLC-DAD. Journal of Chromatographic Science, 2015, 53(4): 478-483. https://doi.org/10.1093/chromsci/bmu068

SHAWKY E., & ABOU EL KHEIR R. M. Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis. Phytochemical Analysis, 2018, 29(5): 452-462. https://doi.org/10.1002/pca.2749

KHDAIR A., MOHAMMAD M. K., TAWAHA K., AL-HAMARSHEH E., ALKHATIB H. S., AL-KHALIDI B., BUSTANJI Y., NAJJAR S., and HUDAIB M. A validated RP HPLC-PAD method for the determination of hederacoside C in Ivy-Thyme cough syrup. International Journal of Analytical Chemistry, 2010, 2010: 478143. https://doi.org/10.1155/2010/478143

GAILLARD Y., BLAISE P., DARRÉ A., BARBIER T., and PÉPIN G. An unusual case of death: suffocation caused by leaves of common ivy (Hedera helix). Detection of hederacoside C, α-hederin, and hederagenin by LC—EI/MS-MS. Journal of Analytical Toxicology, 2003, 27(4): 257-262. https://doi.org/10.1093/jat/27.4.257

PETERSEN M., & SIMMONDS M. S. Rosmarinic acid. Phytochemistry, 2003, 62(2): 121-125. https://doi.org/10.1016/S0031-9422%2802%2900513-7

HADAD G. M., ABDEL SALAM R. A., SOLIMAN R. M., and MESBAH M. K. HPLC–DAD determination of seven antioxidants and caffeine in different phytopharmaceuticals. Journal of Chromatographic Science, 2014, 52(7): 617-623. https://doi.org/10.1093/chromsci/bmt086

ABDALLAH O. M., MOTAAL A. A., and ELBAHRAWY S. A. Development of a Validated Reversed Phase HPLC-UV Method for a Simultaneous Determination of Hederacoside C and Thymol in Ivy-Thyme Cough Syrup. Chemistry Research Journal, 2018, 3(2): 33-41. https://chemrj.org/download/vol-3-iss-2-2018/chemrj-2018-03-02-33-41.pdf

SOLLOHUB K., & CAL K. Spray drying technique: II. Current applications in pharmaceutical technology. Journal of Pharmaceutical Sciences, 2010, 99(2): 587-597. https://doi.org/10.1002/jps.21963

NANDIYANTO A. B. D., & OKUYAMA K. Progress in developing spray-drying methods for the production of controlled morphology particles: From the nanometer to submicrometer size ranges. Advanced Powder Technology, 2011, 22(1): 1-19. https://doi.org/10.1016/J.APT.2010.09.011

TONON R. V., BRABET C., and HUBINGER M. D. Influence of process conditions on the physicochemical properties of açai (Euterpe oleraceae Mart.) powder produced by spray drying. Journal of Food Engineering, 2008, 88(3): 411-418. http://dx.doi.org/10.1016/j.jfoodeng.2008.02.029


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