Deriving Breast Cancer’s Primary Cultures from Patients' Tumor Biopsies in Indonesia Using Explant and Enzymatic Methods
Abstract
Breast cancer is the most common cause of death in women globally with high rates of heterogeneity. The development of breast cancer treatment is still constrained because of the different responses to its therapy. The use of primary breast cancer culture is invaluable because it provides the same properties as breast cancer itself. Primary culture is used as a tool to determine the proliferative ability of breast cancer cells. However, the use of optimum methods in cultivating primary culture must be evaluated because of the unstable nature of primary culture. In this study, we investigate the optimum method for culturing cells derived from the tumor biopsies of breast cancer patients in Indonesia by comparing explant and enzymatic methods. Breast cancer tissues were obtained from five breast cancer patients, who underwent surgery and incision biopsies. Tissues were further cultured by explant and enzymatic methods. The cell cultures were observed daily using a microscope for up to 30 days. The results showed that the cells cultured using the enzymatic method for more than 16 h were susceptible to microbiome contamination in the following days after enzymatic digestion, while those cultured using the explant method grew well for 30 days. The findings of this research suggested that the explant method gave better results compared with the enzymatic method. The findings of this study provide insight into the optimum conditions for the primary culture of breast cancer cells.
Keywords: breast cancer, enzymatic culture, explant culture, in vitro, primary culture.
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SUNG H., FERLAY J., SIEGEL R. L., LAVERSANNE M., SOERJOMATARAM I., JEMAL A., and BRAY F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 2021, 71(3): 209-249. https://doi.org/10.3322/caac.21660
WIDIANA I. K., & IRAWAN H. Clinical and Subtypes of Breast Cancer in Indonesia. Asian Pacific Journal of Cancer Care, 2020, 5(4): 281-285. https://doi.org/10.31557/APJCC.2020.5.4.281-285
DESANTIS C. E., MA J., GODING SAUER A., NEWMAN L. A., and JEMAL A. Breast cancer statistics, 2017, racial disparity in mortality by state. CA: A Cancer Journal for Clinicians, 2017, 67: 439–448. https://doi.org/10.3322/caac.21412
WANG M., ZHAO J., ZHANG L., WEI F., LIAN Y., WU Y., GONG Z., ZHANG S., ZHOU J., CAO K., LI X., XIONG W., LI G., ZENG Z., and GUO C. Role of Tumor Microenvironment in Tumorigenesis. Journal of Cancer, 2017, 8(5): 761-773. https://doi.org/10.7150/jca.17648
KALINOWSKI L., SAUNUS J. M., REED A. E. M., and LAKHANI S. R. Breast Cancer Heterogeneity in Primary and Metastatic Disease. Breast Cancer Metastasis and Drug Resistance, 2019, 1152: 75–104. https://doi.org/10.1007/978-3-030-20301-6_6
LÜÖND F., TIEDE S., CHRISTOFORI G. Breast Cancer as An Example of Tumour Heterogeneity and Tumour Cell Plasticity During Malignant Progression. British Journal of Cancer, 2021, 125(2): 164-175. https://doi.org/10.1038/s41416-021-01328-7
AMALINA N. D., SUZERY M., CAHYONO B., and BIMA D. N. Revealing the Potential of Secondary Metabolites of Indonesian Herbal Plants to Stop Breast Cancer Metastasis: An In-Silico Approach. Indonesian Journal of Chemical Science, 2020, 3(9): 154-159. https://doi.org/10.15294/ijcs.v9i3
JANUŠKEVIČIENĖA I., & PETRIKAITĖ V. Heterogeneity of Breast Cancer: The Importance of Interaction between Different Tumor Cell Populations. Life Science, 2019, 239: 1170092. https://doi.org/10.1016/j.lfs.2019. 117009
TSAI S., MCOLASH L., PALEN K., JOHNSON B., DURIS C., YANG Q., DWINELL M. B., HUNT B., EVANS D. B., GERSHAN J., and JAMES M. A. Development of Primary Human Pancreatic Cancer Organoids, Matched Stromal and Immune Cells and 3D Tumor Microenvironment Models. BMC Cancer, 2018, 18(1): 335. https://doi.org/10.1186/s12885-018-4238-4
GANJIBAKHSH M., AMINISHAKIB P., FARZANEH P., KARIMI A., FAZELI S. A. S., RAJABI M., NASIMIAN A., NAINI F. B., RAHMATI H., GOHARI N. S., MOHEBALI N., ASADI M., GORJI Z. E., IZADPANAH M., MOGHANJOGHI S. M., and ASHOURI S. Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture. Frontiers in Dentistry, 2017, 14(4): 191-202. https://fid.tums.ac.ir/index.php/fid/article/view/1904
ROWEHL R. A., BURKE S., BIALKOWSKA A. B., PETTET D. W. 3RD, ROWEHL L., LI E., ANTONIOU E., ZHANG Y., BERGAMASCHI R., SHROYER K. R., OJIMA I., and BOTCHKINA G. I. Establishment of Highly Tumorigenic Human Colorectal Cancer Cell Line (CR4) with Properties of Putative Cancer Stem Cells. PLoS ONE, 2016, 9(6): e99091. https://doi.org/10.1371/journal.pone.0099091
EHLEN L., ARNDT J., TREUE D., BISCHOFF P., LOCH F. N., HAHN E. M., KOTSCH K., KLAUSCHEN F., BEYER K., MARGONIS G. A., KREIS M. E., and KAMPHUES C. Novel Methods for In Vitro Modeling of Pancreatic Cancer Reveal Important Aspects for Successful Primary Cell Culture. BMC Cancer, 2017, 20: 417. https://doi.org/10.1186/s12885-020-06929-8
HENDIJANI F. Explant Culture: an Advantageous Method for Isolation of Mesenchymal Stem Cells from Human Tissues. Cell Proliferation, 2017, 50(2): e12334. https://doi.org/10.1111/cpr.12334
CIESZYŃSKA M., KLUŹNIAK W., WOKOŁORCZYK D., CYBULSKI C., HUZARSKI T., GRONWALD J., FALCO M., DĘBNIAK T., JAKUBOWSKA A., DERKACZ R., MARCINIAK W., LENER M., WORONKO K., MOCARZ D., BASZUK P., BRYŚKIEWICZ M., NAROD S. A., and LUBIŃSKI J. Risk of Second Primary Thyroid Cancer in Women with Breast Cancer. Cancers, 2021, 14(4): 957. https://doi.org/10.3390/cancers14040957
JI P., ZHANG Y., WANG S. J., GE H. L., ZHAO G. P., XU Y. C., and WANG Y. CD44hiCD24lo Mammosphere-Forming Cells from Primary Breast Cancer Display Resistance to Multiple Chemotherapeutic Drugs. Oncology Reports, 2016, 35(6): 3293-3302. https://doi.org/10.3892/or.2016.4739
KIM D. S., LEE M. W., KO Y. J., CHUN Y. H., KIM H. J., SUNG K. W., KOO H. H., and YOO K. H. Cell Culture Density Affects the Proliferation Activity of Human Adipose Tissue Stem Cells. Cell Biochemistry and Function, 2016, 34(1): 16-24. https://doi.org/10.1002/cbf.3158
KAPAŁCZYŃSKA M., KOLENDA T., PRZYBYŁA W., ZAJĄCZKOWSKA M., TERESIAK A., FILAS V., IBBS M., BLIŹNIAK R., ŁUCZEWSKI Ł., and LAMPERSKA K. 2D and 3D Cell Cultures – A Comparison of Different Types of Cancer Cell Cultures. Archives of Medical Science, 2016, 14(4): 910-919. https://doi.org/10.5114/aoms.2016.63743
EL-SAYAD IBRAHIM S. A. The Role of Media Campaigns in Raising Awareness of Development Issues and Their Relationship to the Level of Anxiety in Adolescents. Journal of Southwest Jiaotong University, 2021, 56(2): 332-349. https://doi.org/10.35741/issn.0258-2724.56.2.27
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