Identification of Sulphur Oxidizing Bacteria on Charcoal Made of Salak Fruit Seeds for Hydrogen Sulfide Removal in Biogas

Retno Ambarwati Sigit Lestari, Supriyono, Ery Fatarina Purwaningtyas, Mega Kasmiyatun

Abstract

Alternative energy sources to substitute fossil fuels have been developed, biogas being one of them. However, H2S needs to be removed in biogas because it promotes corrosion in the equipment using biogas. The H2S can be removed from biogas by biological processes. H2S was removed by biofiltration, in which H2S degrading bacteria immobilized on the packing material inside a column. This study aimed to determine the genera of microorganisms that degrade H2S in biogas. Moreover, this research aimed to investigate the ability of these microorganisms to degrade H2S. The novelty of this research is the use of charcoal from salak fruit seed as a packing material for immobilization the microorganisms in the biofilter; therefore, the packing material is more durable and does not rot. The isolated sludge taken from liquid wastewater treatment in the tofu industry was tested for sulfide degradation. Then, the best of bacteria to degrade sulfide was immobilized on the surface of charcoal made of salak fruit seeds and after acclimatization and the bacteria grew well. We tested their capability of forming biofilms on the surface of the charcoal of salak fruit seeds. Further identification showed that the isolate was Bacillus cereus with a similarity value of 98%. An experiment to remove H2S of biogas using a biofilter column with immobilized Bacillus cereus bacteria on the surface of the charcoal of salak fruit seeds showed that the Bacillus cereus bacteria could degrade H2S of biogas that flew in the biofilter through the surface of charcoal of salak fruit seeds. The highest removal efficiency was obtained for H2S (RE) at the packing height of 80 cm; 97.15% of which was achieved at a biogas flow rate of 30 L/hour, the H2S concentration was 142.48 ppm for 4 hours.

 

Keywords: Bacillus cereus, biogas, biofilm, removal, H2S.

 

https://doi.org/10.55463/issn.1674-2974.49.8.19


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References


HOU N, XIA Y, WANG X, et al. H2S biotreatment with sulfide-oxidizing heterotrophic bacteria. Biodegradation, 2018, 29: 511-524. https://doi.org/10.1007/s10532-018-9849-6

OMAR B, EL-GAMMAL M, ABOU-SHANA R, et al. Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition. Bioresource Technology, 2019, 286: 121413. https://doi.org/10.1016/j.biortech.2019.121413

KHOSHNEVISAN B, TSAPEKOS P, ALFARO N, et al. A review on prospects and challenges of biological H2S removal from biogas with focus on biotrickling filtration and microaerobic desulfurization. Biofuel Research Journal, 2017, 16, 741-750. https://doi.org/10.18331/BRJ2017.4.4.6

NHUT H H, THANH V L T., LE L T. Removal of H2S in biogas using biotrickling filter: Recent development, Process Safety and Environmental Protection, 2020, 144, 297-309. https://doi.org/10.1016/j.psep.2020.07.011

POKORNA-KRAYZELOVA L, MAMPAEYA K E, VANNECKEA T P W, et al. Model-based optimization of microaeration for biogas desulfurization in UASB reactors. Biochemical Engineering Journal, 2017, 125: 171-179. https://doi.org/10.1016/j.bej.2017.06.009

WU H, YAN H, QUAN Y, et al. Recent progress and perspectives in biotrickling filters for VOCs and odorous gases treatment. Journal of Environmental Management, 2018, 222: 409-419. https://doi.org/10.1016/j.jenvman.2018.06.001

RYBARCZYK P, SZULCZYŃSKI B, GĘBICKI J, & HUPKA J. Treatment of Malodorous Air in Biotrickling Filters: A review. Biochemical Engineering Journal, 2019, 141: 146-162. https://doi.org/10.1016/j.bej.2018.10.014

VIKROMVARASIRI N, & PISUTPAISAL N. Hydrogen Sulfide Removal in Biotrickling Filter System by Halothiobacillus Neapolitanus. International Journal of Hydrogen Energy, 2016, 41(35): 15682-15687. https://doi.org/10.1016/j.ijhydene.2016.04.180

LESTARI R A S, SEDIAWAN W B, SYAMSIAH S, SARTO, TEIXEIRA J A. Hydrogen sulfide removal from biogas using a salak fruit seeds packed bed reactor with sulfur oxidizing bacteria as biofilm. Journal of Environmental Chemical Engineering, 2016, 4: 2370-2377. https://doi.org/10.1016/j.jece.2016.04.014

BEN JABER, M, COUVERT, A, AMRANE, A, LE CLOIREC, P, & DUMONT, E. Removal of Hydrogen Sulfide in Air Using Cellular Concrete Waste: Biotic and Abiotic Filtrations. Chemical Engineering Journal, 2017, 319, 268-278. https://doi.org/10.1016/j.cej.2017.03.014

BALTRĖNAS P, BALTRĖNAITĖ E, KLEIZA J. & ŠVEDIENĖ J. A Biochar-Based Medium in the Biofiltration System: Removal Efficiency, Microorganism Propagation and the Medium Penetration Modelling, Journal of the Air & Waste Management Association, 2016, 66(7): 673-686 https://doi.org/10.1080/10962247.2016.1162227

ZHOU, Y, APUL O G, & KARANFIL T. Adsorption of halogenated aliphatic contaminants by graphene nanomaterials. Water Research, 2015, 79, 57-67. https://doi.org/10.1016/j.watres.2015.04.017

QUESADA H B, CUSIOLI L F, DE O BEZERRA C, et al. Acetaminophen Adsorption Using a Low-Cost Adsorbent Prepared from Modified Residues of Moringa Oleifera Lam. Seed Husks. Journal of Chemical Technology and Biotechnology, 2019, 94(10): 3147-3157. https://doi.org/10.1002/jctb.6121

HALL BG. Building phylogenetic trees from molecular data. Molecular Biology and Evolution, 2013, 30: 1229–1235. https://doi.org/10.1093/molbev/mst012

PRAKASH A, SHARMA C, SINGH A, et al. Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and amplified fragment length polymorphism. Clinical Microbiology and Infection 2016, 22: 277.e1-277.e9. https://doi.org/10.1016/j.cmi.2015.10.022

SÄDE E. and BJÖRKROTH J. Identification Methods DNA Fingerprinting: Restriction Fragment-Length Polymorphism, Encyclopedia of Food Microbiology (Second Edition), 2014, 274-281. https://doi.org/10.1016/B978-0-12-384730-0.00410-9

HAOSAGULA S, OAEWC S, PROMMEENATEC P, et al. Profile of sulfur oxidizing bacteria in full-scale Biotrickling filter to remove H2S in biogas from in cassava starch industry. Journal Energy Reports, 2021, 7: 677-685. https://doi.org/10.1016/j.egyr.2021.07.086

WU D, HOU J, WANG X, FENG H, and HE Z. Biological Removal of High Concentration of Hydrogen Sulfide at Low Temperature in a Compost-Based Biofilter. Environmental Engineering, 2021, 24(4): 565-572. https://doi.org/10.6180/jase.202108_24(4).0012

DAS J, RENE E.R, DUPONT C, et al. Performance of a Compost and Biochar Packed Biofilter for Gas-Phase Hydrogen Sulfide Removal. Bioresource Technology, 2019, 273: 581-591. https://doi.org/10.1016/j.biortech.2018.11.052


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