eNOS T786C (rs2070744) Gene Polymorphism and Its Underlying Mechanism Associated with Coronary Heart Disease

Thirunavukkarasu Jaishankar, Meera Shivasekar, V. M. Vinodhini


The study aims to examine plasma nitric oxide concentration and its relationship to T-786C gene polymorphism levels in the development of coronary heart disease in young people. Reduced nitric oxide (NO) bioavailability and endothelial nitric oxide synthase (eNOS) T786C gene polymorphism have been identified as risk factors for the development of coronary heart disease. This cross-sectional study was conducted in SRM Medical College Hospital and Research Centre on 200 angiographically proven CHD subjects attending the Department of Cardiology and medicine and 100 controls from MHC in the age group of ≤ 45 years. Overnight fasting plasma samples were obtained to evaluate lipid profile and nitric oxide by Griess reaction in UV spectrophotometry utilizing the ELISA technique. Polymerase Chain Reaction and Restricted fragment length polymerase amplify the eNOS gene T-786C. As a result, NO levels in plasma were significantly lower in CHD patients than in controls. In addition, C allele carriers of the eNOS T786C polymorphism showed significantly lower mean plasma NO levels than T allele carriers (P < 0.001). Our findings suggest that a lower plasma NO level is related to an increased risk of CHD. Furthermore, the eNOS T786C polymorphism is a significant risk factor for CHD development by lowering NO levels in the plasma. However, the eNOS T786C polymorphism influences the severity of CHD.

Keywords: coronary heart disease, endothelial nitric oxide synthase, gene polymorphism, nitric oxide.




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